Figure 5. DHS increases BAX/BCL2 ratio and induces BID cleavage in IMR32 cells.

(A) Upregulation of BAX expression and BID cleavage and downregulation of BCL2 in the whole cell extract. (B) Translocation of BAX and t-BID into lysosome and mitochondria. The cells were incubated for different periods with DHS (0 and 20 μM) and the whole cell, cytoplasmic, lysosomal and mitochondrial extracts were subjected to immunobloting, using suitable antibodies against the above proteins. The protein bands were detected using a Kodak Gel-doc software and the intensity ratios of the individual bands to that of vehicle control, taken as 1 (arbitrary unit) were quantified after normalizing with respective loading controls. All determinations were made in duplicates in 3-4 different experiments, and the values are mean ± S. E. M. ^*p<0.05 compared to the corresponding vehicle controls. Representative images are shown.