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. 2017 Jul 19;8(43):74263–74275. doi: 10.18632/oncotarget.19392

Figure 6. Effect of annexin A5 on protein kinase c (PKC)-ζ expression.

Figure 6

(A-B) Cells were transfected with annexin A5 siRNA and then treated with auranofin (1 μM) for 24 h. (A) Western blot analysis was performed to detect PKC-ζ expression by using PC-3, LNCaP-LN3, and DU145 whole cell lysates. Quantification of western blots (n = 3). (B) Confocal analysis was performed to detect PKC-ζ expression. (C) PC-3, LNCaP-LN3, and DU145 cells were transfected with annexin A5 siRNA, and qPCR was performed to detect the expression of PKC-ζ mRNA. (D) PC-3 cells were cotransfected with annexin A5 and PKC-ζ siRNA, and western blot analysis was performed to detect COX-2 expression by using the whole cell lysate. Microscopy scale bar = 50 μm. NS = not significant, *P < 0.05.