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MCF-7 cells were seeded at 0.0125x106 cells then were treated with various doses of 17β-estradiol and conjugated 17β-estradiol ranging from 0nM to 50nM for 24h. IGFBP-2 concentrations were determined by ELISA after treatment with 17β-estradiol (A) and conjugated 17β-estradiol (B) The graphs represents the mean±SEM of three independent repeats each conducted in triplicate (* P<0.05, *** P<0.001).
