Figure 7. Role of PTMA overexpression in TGFβ1-induced fibrosis process.

(A and B) The primary human oral submucosa fibroblasts were transfected with pcDNA-PTMA with the absence or presence of TGFβ1 treatment; the cell viability and proliferation of the fibroblast were then determined using CCK-8 and BrdU assays. (C and D) The primary human oral submucosa fibroblasts were transfected with pcDNA-PTMA with the absence or presence of TGFβ1 treatment; the protein levels of PTMA, SMAD4, MMP-9, α-SMA and Collagen I were then determined using Western blot assays. The data are presented as mean ± SD of three independent experiments. **P<0.01, vs PBS + pcDNA group, ## P<0.01, vs TGFβ1 + TPMA group.