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. 2017 Sep 8;8(43):75284–75297. doi: 10.18632/oncotarget.20777

Figure 2. Effects of HSP90AA1-IT1 on the viability of the glioma cells.

Figure 2

The U87MG and U251 glioma cells were infected with lentiviruses expressing small hairpin RNAs of HSP90AA1-IT1 (sh-HSP90AA1-IT1). Lentiviral vector with nonspecific shRNAs was taken as the negative control (NC). (A) The growth curves of the infected glioma cells were shown using CCK8 assay. Data were presented as mean ± s.d. from three independent experiments. (B) Cell proliferations were determined by EDU staining assay. The results represent mean ± s.d. from three independent experiments. ***P < 0.001 vs. NC. (C) Cell populations in G1, S and G2/M phases were determined by flow cytometry. Each bar represents mean ± s.d. from three independent experiments. **P < 0.01 vs. NC, ***P < 0.001 vs. NC. (D) Apoptosis were tested by duel-staining with Annexin V and PI followed by flow cytometry assay. Data were presented as mean ± s.d. from three independent experiments. ***P < 0.001 vs. NC. Scale: 20μm.