Table 2.

Association of S. mutans SpaP subtypes with caries in 35 caries and 35 nearly caries-free extremes of children.

Groupa	SpaP type	Sub-type	DMBT1 binding			DeFS-12yb			DeFS-17yb			ΔDeFS-5yc
			n	Mean ± SD	pd	n	Mean ± SD	pd	n	Mean ± SD	pd	n	Mean ± SD	pd
SpaP A	A		39	13.3 ± 3.6	0.862	39	3.2 ± 4.2	0.518	34	9.0 ± 10.1	0.204	34	5.5 ± 6.5	0.282
	A	A-1	9	13.2 ± 1.7	0.712	9	2.7 ± 4.0	0.882	8	5.3 ± 7.6	0.801	8	3.2 ± 5.2	0.803
	A	C1	10	12.9 ± 1.8	Ref.	10	2.4 ± 3.9	Ref.	9	4.7 ± 7.5	Ref.	9	2.9 ± 5.0	Ref.
SpaP B	B		27	17.8 ± 6.6	0.002	27	3.8 ± 4.4	0.320	22	11.3 ± 5.0	0.114	22	7.3 ± 10.4	0.104
	B	B-1	10	21.8 ± 8.3	< 0.001	10	5.7 ± 4.3	0.074	7	12.3 ± 10.6	0.033	7	8.4 ± 7.3	0.029
	B	B-2	6	19.8 ± 5.3	0.002	6	6.7 ± 3.9	0.041	4	19.0 ± 10.9	0.023	4	12.4 ± 7.2	0.019
	B	C6	2	17.7 ± 2.6	0.053	2	7.0 ± 2.8	0.119	2	28.0 ± 1.4	0.030	2	18.2 ± 0.1	0.029
SpaP C	C	C-1	4	12.8 ± 3.1	0.396	4	2.5 ± 2.5	0.480	3	4.3 ± 2.3	0.299	3	1.0 ± 0.1	0.925
Adhesione
SpaP B	B	High	17	20.7 ± 6.5	< 0.001	17	4.8 ± 4.3	0.073	15	16.1 ± 16.0	< 0.001	15	10.3 ± 11.5	0.003
	B	Low	10	12.8 ± 2.1		10	2.2 ± 4.2		7	1.0 ± 1.2		7	0.9 ± 1.0
SpaP A	A	High	16	16.6 ± 1.5	< 0.001	16	1.9 ± 3.5	0.090	13	6.1 ± 8.0	0.353	13	3.1 ± 3.8	0.260
	A	Low	23	10.9 ± 1.5		23	4.2 ± 4.4		21	10.8 ± 10.9		21	6.9 ± 7.4
Acid tolerancef
SpaP B	B	High				13	4.4 ± 4.6	0.558	13	17.8 ± 16.7	0.001	13	11.6 ± 11.8	0.001
SpaP A	B	Low				14	3.3 ± 4.3		9	2.0 ± 2.2		9	1.1 ± 1.3
	A	High				7	5.3 ± 4.2	0.115	6	12.8 ± 13.1	0.585	6	7.8 ± 9.0	0.820
	A	Low				32	2.8 ± 4.1		28	8.1 ± 9.4		28	4.9 ± 6.0

^aChildren infected with S. mutans SpaP types and subtypes from sequencing and functional tests of isolates in 35 caries and 35 nearly caries-free extremes of children.

^bCaries DeFS (Decayed, enamel included, Filled Surfaces) at 12 and 17 years of age.

^cΔDeFS (5 y) = 5-years prospective caries increment from 12 to 17 years of age.

^d2-sided p-value from Mann-Whitney U test.

^eHighest (50%) and lowest (50%) adhesion phenotypes among the 70 isolates.

^fLow (0) and high (1, 2) acid tolerance.