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. 2017 Oct 24;4(5):ENEURO.0227-17.2017. doi: 10.1523/ENEURO.0227-17.2017

Figure 1.

Figure 1.

D2R-expressing neurons are concentrated in the midline thalamus where D2R expression overlaps with TH innervation in the PVT. In situ hybridization for D2R mRNA shows expression in the PVT and CM thalamus of wild type mice (bregma −1.7 mm; A) but not in D2 knock-out mice (B). Scale bar: 1 mm. C, IHC for GFP (green) in a Drd2-EGFP mouse shows GFP expression in the PVT, IMD, and CM thalamus. Scale bar: 500 µm. D, IHC for GFP (green) and GAD67 (red) in a Drd2-EGFP mouse. No interneurons were found in the PVT. Scale bar: 100 µm. Inset, GAD67 labeling within CA1 of the hippocampus. Scale bar: 100 µm. E, IHC for DAT (green) shows labeling in the MD and lateral habenula (LHb). Scale bar: 250 µm. Inset, DAT (green) labeling in the striatum. Scale bar: 1 mm. F, IHC for TH (red) shows dense innervation of the PVT. Scale bar: 250 µm. Inset, TH (red) labeling in the striatum. Scale bar: 1 mm. DAPI labeling is shown in blue in C–F. PVT, paraventricular nucleus of the thalamus; CM, central medial nucleus of the thalamus; STR, striatum; IMD, intermediodorsal nucleus of the thalamus; LHb, lateral habenula; MD, mediodorsal nucleus of the thalamus.