Article title: “Freezing/Thawing without Cryoprotectant Damages Native but not Decellularized Porcine Renal Tissue”
Authors: Nafiseh Poornejad, Timothy S. Frost, Daniel R. Scott, Brinden B. Elton, Paul R. Reynolds, Beverly L. Roeder, and Alonzo D. Cook
Journal: Organogenesis
Bibliometrics: Volume 11, Issue 1, pages 30–45
DOI: 10.1080/15476278.2015.1022009
The manuscript describes the authors' efforts to improve the processes for decellularization, preservation, and recellularization of porcine kidneys. The authors obtained what were originally labeled as an immortalized cell line of human renal cortical tubular epithelium (RCTE) cells from the Feinberg School of Medicine, Northwestern University to conduct their recellularization experiments. The RCTE cells were later discovered to actually be Madin-Darby Canine Kidney (MDCK) epithelial cells. Despite being of canine origin, MDCK cells are a distal tubule epithelial cell line that behave similarly to human RCTE cells. The conclusions regarding recellularization as reported in the paper are still sound.