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. 2017 Sep 19;8(46):80709–80721. doi: 10.18632/oncotarget.21080

Figure 3. MiR-23a directly targets XIAP 3′UTR.

Figure 3

(A) Predicted binding sequences between miR-23a and seed matches in XIAP-3′UTR. (B) Luciferase reporter analysis of XIAP 3′UTR were performed after co-transfection with miR-23a in T47D cells and after co-transfection with miR-23a ASO in MCF-7 cells. The error bars indicate the standard error of the mean (S.E.M.) for three independent experiments (*P < 0.05). (C) Expression of XIAP inhibits autophagy. Western blot analysis is shown. Vector is used as an internal control. (D) MCF-7 cells were treated with Embelin as indicated and XIAP protein level and LC3-II/I expression were determined by Western blot analysis with anti-XIAP and anti-LC3, respectively. (E) Western blot. T47D cells were grown and transfected with miR-23a mimics, miR-23a mimics plus vector, miR-23a mimics plus expression of XIAP, or negative control. Then, total cellular proteins from these cells were subjected to Western blot analysis of XIAP, LC3 expression. (F) Western blot. Forty-eight hours after transfected with miR-23a mimics and Negative control in T47D cells, which were treated with 20 uM Z-VAD-FMK or DMSO for 2 h. Cell lysates were analyzed by Western blotting.