Antibodies |
|
Anti-mouse CD3 (clone 145-2C11) |
Biolegend |
100306 |
Anti-mouse CD4 (clone RM4.5) |
Biolegend |
100566 |
Anti-mouse CD5 (clone 53-7.3) |
Biolegend |
100606 |
Anti-mouse CD11b (clone M1/70) |
Biolegend |
101224 |
Anti-mouse CD11c (clone N418) |
Biolegend |
117312 |
Anti-mouse CD19 (clone 6D5) |
Biolegend |
11506 |
Anti-mouse CD25 (clone PC61) |
Biolegend |
102038 |
Anti-mouse CD45 (clone 30-F11) |
Biolegend |
103128 |
Anti-mouse CD49b (clone DX5) |
eBioscience |
11-5971-85 |
Anti-mouse CD127 (clone A7R34) |
Biolegend |
135013 |
Anti-mouse ICOS (clone 15F9 |
eBioscience |
46-9940-82 |
Anti-mouse GR1 (clone RB6-8C5) |
Biolegend |
108406 |
Anti-mouse IL-5 (clone TRFK5 |
Biolegend |
504304 |
Anti-mouse IL-13 (clone eBio13A) |
eBioscience |
25-7133-82 |
Anti-mouse Ly6G (clone 1A8) |
Biolegend |
127616 |
Anti-mouse SiglecF (clone ES22-10D8) |
Miltenyi |
130-102-274 |
Anti-mouse ST2 (clone RMST2-2) |
eBioscience |
17-9335-82 |
Anti-mouse TER119 (clone TER-119) |
Biolegend |
116220 |
Anti-HMGB-1 rabbit polyclonal |
Abcam |
Ab18256 |
Anti-c-myc (clone Myc.A7) |
Thermo Fisher Scientific |
MA1-21316 |
Anti-human IL-33 goat polyclonal |
R&D Systems |
AF3625 |
Anti-mouse IL-33 goat polyclonal |
R&D Systems |
AF3626 |
Anti-mouse IL-1α |
R&D Systems |
AF-400-NA |
IgG1 isotype control antibody (clone MOPC-21) |
Produced in-house |
N/A |
|
Bacterial and Virus Strains |
|
Heligmosomoides polygyrus |
(Johnston et al, 2015) |
N/A |
Nippostrongylus brasiliensis |
(Lawrence et al, 1996) |
N/A |
|
Biological Samples |
|
Human lung tissue |
Lothian NRS Bioresource |
15/ES/0094 |
|
Chemicals, Peptides, and Recombinant Proteins |
|
Recombinant mouse IL-1α |
Biolegend |
575002 |
Recombinant mouse IL-33 |
Biolegend |
580506 |
Recombinant human IL-33 |
Biolegend |
581806 |
ST2-Fc |
Biolegend |
557904 |
Dynabeads Protein G |
Thermo Fisher Scientific |
10004D |
Proteinase K |
Sigma |
557904 |
DNAse (protease-free) |
Sigma |
4536282001 |
Liberase TL |
Sigma |
05401020001 |
Methylcholine chloride |
Sigma |
A2251 |
Hoescht 33342 |
Thermo Fisher Scientific |
H3570 |
|
Critical Commercial Assays |
|
Mouse IL-33 Duoset ELISA |
R&D systems |
DY3626 |
Human IL-33 Duoset ELISA |
R&D systems |
DY3625B |
Annexin V Apoptosis Detection Kit |
eBioscience |
88-8005-72 |
Limulus Amoebocyte Lysate assay |
Lonza |
QCL-1000 |
|
Experimental Models: Cell Lines |
|
HEK293T |
ATCC |
CRL-3216 |
CMT-64 |
ECACC |
10032301 |
|
Experimental Models: Organisms/Strains |
|
Mouse: IL-13-eGFP (C57BL/6J) |
(Neill et al., 2010) |
N/A |
Mouse: hIL-33+/+ / mIL-33–/– (humanized IL-33) (BALB/c) |
(Cohen et al., 2015) |
N/A |
|
Recombinant DNA |
|
pSecTAG2A plasmid |
Thermo Fisher Scientific |
V90020 |
Software and Algorithms |
ClustalX |
(Thompson et al., 1997) |
www.clustal.org |
Mascot v2.4 |
Matrix Science |
www.matrixscience.com |
SMART |
(Letunic et al., 2015) |
smart.embl-heidelberg.de/ |
HHpred |
(Söding, 2005) |
toolkit.tuebingen.mpg.de/#/tools/hhpred |
Modeller v9.12 |
(Sali and Blundell, 1993) |
salilab.org/modeller/ |
APBS |
(Baker et al, 2001) |
www.poissonboltzmann.org/ |
ESPript v3 |
(Robert and Gouet, 2014) |
espript.ibcp.fr/ |
PyMOL |
Schrödinger, LLC |
www.pymol.org |
PROSITE |
(de Castro et al., 2006) |
prosite.expasy.org/ |
Protein Data Bank |
(Berman et al., 2000) |
www.rcsb.org/pdb |
Wormbase ParaSite |
(Howe et al., 2016) |
parasite.wormbase.org/ |
FlowJo v9.1 |
Flowjo, LLC |
www.flowjo.com/ |
Prism v7 |
Graphpad Software |
www.graphpad.com/scientific-software/prism/ |
BIAcore T200 software v2.01 |
GE Healthcare |
N/A |
|
Other |
|
Superdex 200 10/300 GL |
GE Healthcare |
17517501 |
MonoQ 5/50 GL |
GE Healthcare |
17-5166-01 |
Series S Sensor Chip NTA |
GE Healthcare |
BR-1005-32 |