Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Kinetic Parameters of PtpA and the Chemically Derived Mutants PtpADha53 and PtpADha53Dha16Dha11
| PtpA Isoform | vmax (U mg−1) | kcat (s−1) | KM (mM) | kcat/KM (105 M−1 s−1) |
|---|---|---|---|---|
| Cys53Cys16Cys11 | 35 ± 0.8 | 11 ± 0.2 | 0.80 ± 0.06 | 1.4 |
| Dha53Cys16Cys11 | 36 ± 1.5 | 12 ± 0.5 | 0.96 ± 0.13 | 1.2 |
| Dha53Dha16Dha11 | 3.8 ± 0.2 | 1.2 ± 0.1 | 0.98 ± 0.12 | 0.12 |
| Cys53Cys16Cys11 + GSNO | 24 ± 1.2 | 8 ± 0.4 | 1.5 ± 0.2 | 0.53 |
| Dha53Cys16Cys11 + GSNO | 39 ± 0.9 | 13 ± 0.3 | 0.84 ± 0.07 | 1.5 |
