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. 2014 Jan 1;14:272. doi: 10.1093/jisesa/ieu134

Infection With the Secondary Tsetse-Endosymbiont Sodalis glossinidius (Enterobacteriales: Enterobacteriaceae) Influences Parasitism in Glossina pallidipes (Diptera: Glossinidae)

Florence N Wamwiri 1,2, Kariuki Ndungu 1, Paul C Thande 1, Daniel K Thungu 1, Joanna E Auma 1, Raphael M Ngure 3
PMCID: PMC5657924  PMID: 25527583

Abstract

The establishment of infection with three Trypanosoma spp (Gruby) (Kinetoplastida: Trypanosomatidae), specifically Trypanosoma brucei brucei (Plimmer and Bradford), T. b. rhodesiense (Stephen and Fatham) and T. congolense (Broden) was evaluated in Glossina pallidipes (Austen) (Diptera: Glossinidae) that either harbored or were uninfected by the endosymbiont Sodalis glossinidius (Dale and Maudlin) (Enterobacteriales: Enterobacteriaceae). Temporal variation of co-infection with T. b. rhodesiense and S. glossinidius was also assessed. The results show that both S. glossinidius infection ( χ2  = 1.134, df = 2, P  = 0.567) and trypanosome infection rate ( χ2  = 1.85, df = 2, P  = 0.397) were comparable across the three infection groups. A significant association was observed between the presence of S. glossinidius and concurrent trypanosome infection with T. b. rhodesiense ( P  = 0.0009) and T. congolense ( P  = 0.0074) but not with T. b. brucei ( P  = 0.5491). The time-series experiment revealed a slight decrease in the incidence of S. glossinidius infection with increasing fly age, which may infer a fitness cost associated with Sodalis infection. The present findings contribute to research on the feasibility of S. glossinidius -based paratransgenic approaches in tsetse and trypanosomiasis control, in particular relating to G. pallidipes control.

Keywords: Glossina, Sodalis glossinidius, trypanosome, co-infection, vector competence

Glossina pallidipes (Austen) (Diptera: Glossinidae) is one of the most important tsetse fly vectors in Eastern Africa because of its widespread distribution ( Ouma et al. 2011 ). This species has been implicated in the spread of Human African Trypanosomiasis and is a key vector for animal trypanosomes in this region ( Ohaga et al. 2007 , Malele et al. 2011 , Peacock et al. 2012 ). Tsetse flies are considered to be naturally refractory to trypanosome infection and only a few of the trypanosomes introduced into a fly via an infective feed are able to overcome the immune system response and thus establish an infection ( Welburn and Maudlin 1999 ). The establishment and maturation of trypanosomes in the tsetse gut is dependent on many variables and involve complex interactions between the fly, endosymbionts, and the parasite itself ( Welburn and Maudlin 1999 ). Tsetse flies harbor at least three gut endosymbionts namely; the obligate Wigglesworthia glossinidae , the facultative Sodalis glossinidius and the ricketssia-like Wolbachia pipientis ( Cheng and Aksoy 1999 ). Wigglesworthia and S. glossinidius are transmitted through maternal milk gland secretions to the intra-uterine developing larva, whereas Wolbachia is transmitted transovarially. Symbionts are therefore present at eclosion in the teneral fly, whereas trypanosome infection is acquired mainly at the first feed in the presence of an infected blood meal source. In various arthropods, gut microbiota have been shown to increase insect immunity to pathogens such as viruses and parasites ( Teixeira et al. 2008 , Moreira et al. 2009 , Koch and Schmid-Hempel 2011 ). In the adult tsetse fly, responses to parasite infection are indirectly modulated by symbionts ( Weiss et al. 2013 ). The possible influence of secondary symbionts on tsetse vectorial capacity has been investigated primarily using homogeneous laboratory populations of Glossinamorsitansmorsitans ( Rio et al. 2006 ) and Glossinapalpalis gambiensis ( Geiger et al. 2007 ), whereby all individuals are S. glossinidius infected. This study utilized by using a naturally heterogeneous population of G. pallidipes to establish the correlation between S. glossinidius infection in G. pallidipes and experimental infection with T. b. brucei , T. b. rhodesiense , and T. congolense. The temporal variation of T. b. rhodesiense and S. glossinidius co-infection was also investigated.

Materials and Methods

Infection of Tsetse Flies

Male teneral G. pallidipes of age 0–2-d-old from the Trypanosomiasis Research Centre (TRC) colony were used. Details of the three trypanosome isolates used for the fly infection are presented in Table 1 . The stabilates were expanded in two donor Swiss white mice that had been immune-suppressed with cyclophosphamide at a dose of 300 mg/kg body weight. Disease progression in the mice was monitored by collection and microscopic examination of blood obtained through tail snipes on alternate days. At the peak of parasitemia, the mice were euthanized using concentrated carbon dioxide. Blood from the heart was then collected by cardiac puncture into a tube containing ethylene diamine tetra acetic acid (EDTA). The level of parasitemia was estimated using the matching method ( Herbert and Lumsden 1976 ) and subsequently, an inoculum dose of 1 × 10 6 trypanosomes/ml was prepared in phosphate saline glucose pH 8.0. Two milliliters of this inoculum used to infect 12 recipient mice. At peak parasitemia, teneral flies in 4″ diameter cages were allowed to feed on the belly of the infected mice. Feeding success was confirmed by visual observation of engorged fly abdomens. Flies that did not feed were excluded from the experiment. After 10–15 min, feeding was interrupted and the engorged flies transferred to the insectary which is maintained at a temperature of 24 ± 1°C and 70 ± 5% relative humidity. These flies were fed on defibrinated bovine blood on alternate days using the in vitro feeding system ( Feldmann 1994 ).

Table 1.

Trypanosome isolates used to infect teneral G. pallidipes

Parasite T. b. rhodesiense T. b. brucei T. congolense
Isolate code KETRI2537 KETRI3386 EATRO993
Host Human G. pallidipes G. pallidipes
Origin, year of isolation Busoga, 1972 Kibwezi, 1979 South Nyanza, 1962
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Experimental Design

Experimental flies were assigned to four infection groups: (1) T. b. rhodesiense infection time series experiment (TBR ts ), (2) T. b. rhodesiense infection (TBR 35 ), (3) T. b. brucei infection (TBB), and (4) T. congolense (TC) infection groups were constituted as detailed in Table 2 . The assays for groups 2–4 were conducted after completion of the respective trypanosome maturation period.

Table 2.

Details of the experimental groups used

Group no. Experimental group N Parasite Dissection performed at dpi
1 TBR ts 100 (25, 22, 26, 27) T. b. rhodesiense 7, 14, 21, 28
2 TBR 35 18 T. b. rhodesiense 35
3 TBB 77 T. b. brucei 40
4 TC 98 T. congolense 30
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TBR, T. b. rhodesiense ; TBB, T. b. brucei ; TC, T. congolense ; dpi, days post-infection; TBR ts , TBR infection time series experiment; TBR 35 , TBR infection with dissection at 35 dpi; numbers of n in brackets represent number of flies dissected the different time points of 7, 14, 21, and 28 dpi for the group TBR ts .

Dissections and DNA Extraction

Dissections were performed on a microscope slide using phosphate buffered saline (137 mM NaCl, 2.7 mM KCl, 4.3 mM Na 2 HPO 4 , 1.47 mM KH 2 PO 4 , pH 7.4). Following the method of Lloyd et al. (1924) , the mouthparts, gut, and the salivary glands (in the brucei infection groups only) were isolated and examined microscopically. Subsequently, individual midguts from the dissected tsetse flies were placed in a 1.5-ml microfuge tube. Total genomic DNA was isolated from the midgut samples using the DNeasy Blood and Tissue Kit (Qiagen Sciences, Gaithersburg, MD, USA), with a minor modification to the manufacturer’s instructions being that the final elution step was performed with 50 µl instead of 100 µl of elution buffer.

PCR Detection of Trypanosome and S. glossinidius Infections

Midgut trypanosome infection in the T. congolense treatment group was determined using the primers specific for T. congolense savannah previously described by Masiga et al. (1992) while infections in the T. b. brucei infection groups were determined using T. b. rhodesiense specific primers (TBR) 1 and TBR2 ( Moser et al. 1989 ). The presence of S. glossinidius in the gut tissues was determined using the primers GPO1 F/R which amplify a 1.2-kb product of the extra-chromosomal plasmid ( Dale and Maudlin 1999 ). The 20 -μl final PCR reaction contained 2 μl of 10× PCR reaction buffer, 2.5 mM MgCl 2 , 0.5 mM dNTPs, 500 nM of each primer, and 0.3 μl of GoTaq Flexi DNA polymerase 5 units/μl (Promega, Madison, WI, USA). For each PCR run, a negative control (water) and the respective positive controls were included. After completion of the PCR run, 10 μl of the amplification products was analyzed by electrophoresis in TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH 8.0) on a 1.5% agarose gel together with a 100-bp DNA ladder size standard (Invitrogen, Carlsbad, CA, USA) and visualized using ethidium bromide staining.

Statistical Analysis

Fisher’s exact test was used for analysis of categorical data using the online program GraphPad found at http://www.graphpad.com/quickcalcs/contingency1.cfm .

Results

S. glossinidius Infection

S . glossinidius was detected in 38.9, 36.4, and 32.7% of the TBR 35 , T. b. brucei , and T. congolense group flies, respectively. There was no significant difference in S. glossinidius prevalence among these three groups ( χ2  = 1.134, df = 2, P  = 0.567). However, S. glossinidius infection prevalence in the TBR ts was higher and ranged between 65 and 86% depending on the period after the infective blood meal, with an average infection rate of 76.5 ± 8.9%.

Trypanosome Infection

Dissections of TBR ts group flies detected three midgut infections, two of which were identified at 7-d postinfection (dpi) and one at 14 dpi ( Table 3 ). No parasites were observed microscopically in the TBR 35 treatment group. In the T. b. brucei group, 7.8% ( n  = 77) of the dissected flies were infected with trypanosomes in the mouthparts, the midgut, or both. No salivary gland infections were observed, indicating the absence of mature infections. In total, 13.3% ( n  = 98) of T. congolense experimental group flies dissected harbored trypanosomes. In the latter group, out of 13 infected flies, 12 had parasites in both mouthparts and the midgut, whereas only 1 fly had an immature infection with no trypanosomes found in the midgut. PCR analysis detected 74% trypanosome infection rate in the TBR ts group, but only 50% infections at 35 dpi (TBR 35 group). Trypanosome infection was detected in 52 and 61% of the T. b. brucei and T. congolense treatment groups, respectively. Comparing infection rates at trypanosome maturity, these were not significantly different between the TBR 35 , TBB, and TC infection groups as determined by both dissection ( χ2  = 3.62, df = 2, P  = 0.163) and PCR methods ( χ2  = 1.85, df = 2, P  = 0.397).

Table 3.

Trypanosome infections in G. pallidipes

Parameter T. b. rhodesiense ts T. b. rhodesiense 35 T. b. brucei T. congolense
Dissection 3/100 (3) 0/18 (0) 6/77 (7.8) 13/98 (13.3)
Mature infections a 0/3 (0) 0/0 (0) 0/6 (0) 12/13 (92.3)
Trypanosome infection (PCR) 74/100 (76) 9/18 (50) 40/77 (51.9) 60/98 (61.2)
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a Number of mature trypanosome infections out of total infections; Percentages are in brackets; T. b. rhodesiense ts TBR infection time series experiment; T. b. rhodesiense35 TBR infection with dissection at 35 dpi.

S. glossinidius and Trypanosome Co-infection

T. b. rhodesiense Infection Group

The temporal variation of parasite and Sodalis infections in the time-series TBR ts experiment is detailed in Table 4 . An apparent decrease in the prevalence of S. glossinidius with increasing number of dpi and hence with the age of the assayed flies ( r  = −0.56) was noted. This may infer a negative fitness cost associated with Sodalis infection, whereby infected flies have reduced longevity.

Table 4.

Temporal variation of T. b. rhodesiense and Sodalis infections in G. pallidipes midgut

Dpi n S+ T+ S+T− S−T− S+T+ S−T+ P -value
7 25 20 (80.0) 15 (60.0) 6 (24.0) [30] 4 (16.0) 14 (56.0) [70.0] 1 (4.0) 0.1206
14 22 19 (86.4) 16 (72.7) 4 (18.2) [21.1] 2 (9.1) 15 (68.2) [78.9] 1 (4.5) 0.1688
21 26 17 (65.4) 18 (69.2) 3 (11.5) [17.6] 5 (19.2) 14 (53.8) [82.4] 4 (15.4) 0.0781
28 27 20 (74.1) 25 (96.6) 0 (0) [0] 2 (7.4) 20 (74.1) [100] 5 (18.5) 0.0598
Total 100 76 (76) 74 (74) 13 (13) [17.1] 13 (13) 63 (63) [82.9] 11 (11) 0.0009*
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dpi, days post infection; n , number of flies tested; S+, total number of flies harboring symbiont; T+, total number of trypanosomes infected flies; S+T+, flies with both Sodalis and trypanosome infection; S+T−, Sodalis- infected without parasite; S−T+, parasite infected flies lacking Sodalis ; S−T−, flies with neither symbiont nor parasite. % prevalence indicated in brackets calculated with reference to total flies at the specific time period after infection. Values in square brackets represent parasite prevalence calculated with reference to the corresponding number of Sodalis -infected flies (s+). P -value, Fisher’s exact test for association between Sodalis and trypanosome infection. *Statistically significant P  < 0.01.

In the T. b. rhodesiensets group, 76% of flies were infected with the symbiont and out of these, 82.9% had established the trypanosome infection by 28 dpi. In contrast, only 45.8% of those flies lacking the symbiont (S − T + ) were able to establish trypanosomes by this time point ( Table 5 ). Overall, in this group there was a highly significant association between infection with S. glossinidius and T. b. rhodesiense infection ( P  = 0.0009, Fisher’s exact test). Analysis of temporal infection reveals that at 7, 14, 21, and 28 dpi, the proportion of S. glossinidius -positive flies that were infected by trypanosome parasites (S + T + ) was constantly higher (76.5 ± 8.9%) than those in which the parasite did not establish (S + T − ) (17.9 ± 6.3%). Infection prevalence in the TBR 35 group was 38.9% for S. glossinidius and 50% for trypanosomes. In this group as well, this association was statistically significant ( P  = 0.0023).

Table 5.

Sodalis and parasite co-infection in experimentally infected G. pallidipes

TBR ts ( n  = 100)
TBR 35 ( n  = 18)
TBB ( n  = 77)
TC ( n  = 98)
T+ T− T+ T− T+ T− T+ T−
S+ 63 (63%) [82.9] 13 (13%) 0 (0%) [0] 7 (38.9%) 8 (10.4) [61.5] 5 (6.5) 26 (26.5%) [ 81.3] 6 (6.1%)
S− 11 (11%) 13 (13%) 9 (50%) 2 (11.1%) 32 (41.6) 32 (41.6) 34 (34.7%) 32 (32.7%)
P -value P  = 0.0009 P  = 0.0023 P  = 0.5491 (NS) P  = 0.0074
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TBR ts , TBR infection time series experiment; TBR 35 , TBR infection with dissection at 35 dpi; TBB, T. b. brucei ; TC, T. congolense ; S+/−, Sodalis positive/negative; T+/−, trypanosome positive/negative; NS, not significant Fisher’s exact test. Bold values in square brackets indicates % of Sodalis- positive flies that were also parasite-positive.

T. b. brucei and T. congolense Infection Groups

In S. glossinidius -infected flies, an infection rate of 61.5 and 74.3% was detected with T. b. brucei and T. congolense , respectively. In comparison, with S. glossinidius -negative flies, infection rates of 50 and 53.1% were detected with T. b. brucei and T. congolense , respectively. Fisher’s exact test revealed that the association between the presence of S. glossinidius and concurrent trypanosome infection was statistically significant in T. b. rhodesiense and T. congolense but not in T. b. brucei ( Table 5 ).

Discussion

This study provides a significant insight into the contribution of endosymbiont S. glossinidius to the outcome of exposing tsetse flies to trypanosome-infected mice. Tsetse flies reproduce by adenotrophic viviparity, whereby the developing larva is nourished in utero by secretions from the milk gland. It is through these secretions that S. glossinidius is transferred from the mother to offspring ( Balmand et al. 2013 ). S. glossinidius is therefore present at eclosion of the teneral fly, whereas trypanosomes are ingested by the fly at its first and/or subsequent infective feeds ( Welburn and Maudlin 1992 ). Previous researchers have fed flies with various antibiotics to eliminate gut endosymbionts before performing similar comparative experiments ( Weiss et al. 2013 ). However, antibiotic treatment has been shown to have negative effects on fly fecundity and longevity ( Alam et al. 2011 ) and may ultimately have some effect on the development of trypanosome infection. In this study, we had access to a laboratory population of G. pallidipes that was naturally heterogeneous with respect to S. glossinidius infection, thereby eliminating the need for antibiotic treatment.

The S. glossinidius prevalence of the time series group (TBR ts ) was 76%, whereas for the TBR 35 , TBB, and TC groups, it was ∼35%. This variation could be because of the fact that while flies in the last three groups were assayed when they were the same age, the time-series group was actually a composite group composed of four distinct groups that were assayed sequentially at different ages. In this study, we noted a negative correlation between S. glossinidius prevalence and the age of the specific group. This may have contributed to the much higher prevalence detected in the relatively younger composite group. It has been shown that although the relative density of S. glossinidius in individual flies may vary with age, the infection is permanent and is not lost in the tsetse’s lifetime ( Maudlin 1991 , Rio et al. 2006 ). We therefore surmise that this apparent temporal decrease in prevalence is not due to reduced density or total symbiont loss, but is a complete absence of the bacteria . This result introduces the hypothesis that S. glossinidius infection may affect tsetse longevity. This phenomenon has been reported in other arthropods such as the pea aphid whereby the secondary symbionts Hamiltonella , Regiella , and Spiroplasma have caused negative effects on host longevity and fecundity ( Maudlin 1991 ). A similar scenario in tsetse flies would add a new angle to the proposed use of S. glossinidius -based paratransgenic approaches in tsetse fly control. We intend to conduct further research to verify the effects, if any, of S. glossinidius infection on longevity of G. pallidipes .

In our study, a significant association between the presence of S. glossinidius and concurrent trypanosome infection was noted in T. b. rhodesiense and T. congolense but not in T. b. brucei . The findings related to the last parasite may be considered to be anomalous, given that the synergistic effect of S. glossinidius on trypanosome establishment and maturation is hypothesized to apply to trypanosome species that pass through a midgut stage in the fly including T. congolense, T. b. brucei , and Trypanosomasimiae but excluding Trypanosomavivax ( Welburn et al. 1993 ). The results we obtained agree with previously reported findings which postulate that S. glossinidius infection decreases the susceptibility of wild tsetse to infection with various trypanosomes ( Farikou et al. 2010 ). However, the findings deviate from other studies which found no correlation between S. glossinidius infection and the ability of G. p. gambiensis to acquire T. congolense ( Geiger et al. 2005 ). Studies using natural populations often reach contradictory conclusions, mainly because of the highly variable levels of S. glossinidius infection in wild flies. This prevalence varies depending on species and even populations, from apparently absent in Glossinafuscipes fuscipes ( Lindh and Lehane 2011 , Alam et al. 2012 ) to more than 50% in Glossinapalpalis palpalis ( Farikou et al. 2010 ). In the latter, S. glossinidius was detected in ∼55% of flies analyzed, and 59% of these were coinfected with various trypanosomes, primarily T. congolense and T. b. brucei sub-species ( Farikou et al. 2010 ). Although a strong corelation was shown in G. p. palpalis , no corelation was noted between infection with S. glossinidius and trypanosome establishment in Kenyan G. austeni and G. pallidipes with less than 2% of ∼600 samples analyzed harboring both S. glossinidius and trypanosomes ( Wamwiri et al. 2013 ). It is however evident that flies without S. glossinidius infection are also capable of developing trypanosome infections ( Alam et al. 2012 ) as well. These divergent conclusions highlight the considerable influence of vector–trypanosome species pairings on the success of infection establishment ( Moloo et al. 1992 ).

This study reinforces the current opinion that concurrent S. glossinidius infection increases susceptibility to trypanosome infection; however, the extent of this effect is depends on the fly species and parasite involved. We also postulate that S. glossinidius infection may have a negative effect on longevity in G. pallidipes , which could have important implications for the application of S. glossinidius -based tsetse control interventions. However, a greater understanding of the interplay between the effects of S. glossinidius infection on fly survival and trypanosome-susceptibility is required.

Acknowledgments

The authors thank members of the IAEA-CRP on Tsetse Symbiosis for valuable discussions on this subject and Patrick Abila for useful comments on the manuscript. The technical assistance of Purity Gitonga, James Murage, Patrick Obore and George Kimotho is gratefully acknowledged. This work was supported by the International Atomic Energy Agency (IAEA) Coordinated Research Project “Improving SIT for Tsetse Flies through Research on their Symbionts and Pathogens” (Contract No.14134).

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