Krall BE, Wang B, Munoz DM, Ilic N, Raghavan S, Niederst MJ, Yu K, Ruddy DA, Aguirre AJ, Kim JW, Redig AJ, Gainor JF, Williams JA, Asara JM, Doench JG, Janne PA, Shaw AT, McDonald III RE, Engelman JA, Stegmeier F, Schlabach MR, Hahn WC. 2017. KEAP1 loss modulates sensitivity to kinase targeted therapy in lung cancer. eLife 6:e18970. doi: 10.7554/eLife.18970.
Published 1, February 2017
In the published article, there is an error in the inline table for sgRNA sequences—the “Target sequence” for sgKEAP1-4is shown as “GAGGACACACTTCTCGCCCA”, which is a duplicate of the target sequence for sgKEAP1-3. The correct “Target sequence” for sgKEAP1-4is ACTGGGCGGCCGGTGCATCC.
The Corrected Table is shown here:
sgRNA Sequences
| Name | Target Sequence |
|---|---|
| sgGFP | GGCGAGGGCGATGCCACCTA |
| sgKEAP1-1 | CTTGTGGGCCATGAACTGGG |
| sgKEAP1-2 | TGTGTCCTCCACGTCATGAA |
| sgKEAP1-3 | GAGGACACACTTCTCGCCCA |
| sgKEAP1-4 | ACTGGGCGGCCGGTGCATCC |
| sgLACZ-1 | AACGGCGGATTGACCGTAAT |
| sgLACZ-2 | CTAACGCCTGGGTCGAACGC |
The originally published Table is also shown for reference:
sgRNA Sequences
| Name | Target Sequence |
|---|---|
| sgGFP | GGCGAGGGCGATGCCACCTA |
| sgKEAP1-1 | CTTGTGGGCCATGAACTGGG |
| sgKEAP1-2 | GAGGACACACTTCTCGCCCA |
| sgKEAP1-3 | GAGGACACACTTCTCGCCCA |
| sgKEAP1-4 | GAGGACACACTTCTCGCCCA |
| sgLACZ-1 | AACGGCGGATTGACCGTAAT |
| sgLACZ-2 | CTAACGCCTGGGTCGAACGC |
The article has been corrected accordingly.