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. 2017 Sep 20;8(48):84595–84609. doi: 10.18632/oncotarget.21102

Figure 4. Comparison of WT ATF5 with dnATF5 Variants.

Figure 4

WT ATF5 contains a basic DNA binding domain adjacent to the leucine zipper domain. The first generation of dnATF5, i.e. Azip, was about a third of the molecular weight of WT ATF5, and had the N-terminal portion and the DNA binding domain removed, accompanied by an acidic enhanced leucine zipper and an N-terminal FLAG tag. This further led to the development of recombinant ATF5 which had the C-terminal portion of Azip removed and included the addition of an N-terminal penetratin motif. The synthetic variant of this cell-penetrant dnATF5 protein was further modified by removal of the FLAG tag and a segment of residual vector-derived amino acids. Image was generated using PowerPoint 2013 [80].