Figure 2.

Bortezomib inhibits the nuclear factor-kappa B signaling pathway in blastic plasmacytoid dendritic cell neoplasm cell lines and primary cells. (A–B) BPDCN cell lines (GEN 2.2 and CAL-1 cells, n=3) and primary BPDCN cells from a patient were incubated with bortezomib (50 nM and 75 nM) or vehicle for 6 h before TLR7 stimulation for 45 min (R848, 1 μg/mL). One representative example of intracellular expression of NF-κBp-65 evaluated in CAL-1, GEN 2.2 cell lines and in primary BPDCN cells from patient #66 were analyzed by (A) flow cytometry and by (B) confocal microscopy in the CAL-1 cell line.