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. 2017 Jul 7;6(10):e1346765. doi: 10.1080/2162402X.2017.1346765

Figure 3.

Figure 3.

DMXAA injection induces a dynamic immune cell recruitment involving both innate and adaptive cells. A. Neutrophils are scarce in growing tumors (top), and abundant 24 hours after DMXAA injection (bottom). B. Kinetics of immune cell recruitment measured by flow cytometry reveals a high influx of neutrophils (CD11b+ Ly6C− Ly6G+ F4/80−) during the first 24h, followed by monocytes (CD11b+ Ly6C+ Ly6G− F4/80+) (day4) and CD8 T cell (day4–8) recruitment. DMXAA also induced a decrease of TAM (CD11b+ Ly6C− Ly6G− F4/80+). n = 10–20 tumors by time point and x = 4 independent experiments. Mann-Whitney test was used for * (p < 0.1), ** (p < 0.01) and *** (p < 0.001) in this and the following figures. C. Localization of immune cells in the tumor after DMXAA injection. Immunofluorescence staining was performed on tumor slices (350µm thick) at different time points after DMXAA injection (day 0 (ctrl), day 4 and day8). Before DMXAA injection (top), tumors showed a limited infiltrate of immune cells mostly located in stromal areas (fibronectin+) whereas after DMXAA injection (middle and bottom), immune cells also invaded EpCam+ tumor islets.