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. 2017 Jul 28;8(45):78480–78495. doi: 10.18632/oncotarget.19652

Figure 4. Silencing HO-1 gene expression augmented cell cycle arrest (G0/G1) induced by SAHA in LY-10 cells.

Figure 4

(A) LY-10 cells were grouped into LY-10 (control), LY-10 (vector 1), LY-10 (HO-1), LY-10 (vector 2), and LY-10 (siHO-1). Different groups of LY-10 cells were treated with SAHA (8 μM) for 24 h. Cell cycle was analyzed by flow cytometry. (B) Western blot of cell cycle proteins in different groups of LY-10 cells. Protein expressions of HO-1, P27, CDK2, CDK4, cyclin D1, and cyclin E1 were detected. Data were analyzed with Prism V5.0 (GraphPad Software, San Diego, CA, USA). Each sample was normalized by related β-actin. All experiments were repeated three times. *P<0.05, **P<0.01.