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. 2017 Oct 9;6(10):e387. doi: 10.1038/oncsis.2017.85

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Copyright © 2017 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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RHD domain of p65 carries HSC70 recognition motifs. (a) Bacterially expressed GST-p65 was incubated with His-HSC70, followed by pull down with GSH beads. The precipitates were detected for proteins as indicated. (b) Upper panel: schematic illustrations of p65 fragments. Lower panel: Flag-p65-full-length (FL), N-terminus (p65-N) or C-terminus (p65-C) was transfected into HEK293T cells. And cell lysates were incubated with recombinant GST-HSC70 followed by pull down with GSH beads. (c) Upper panel: schematic illustrations of HSC70 fragments. Lower panel: Flag-HSC70-FL/N/C was transfected into HEK293T cells. Cell lysates were incubated with recombinant GST-p65-N followed by GST-pull down and immunoblots analysis. (d) Identification for putative KFERQ motifs within the RHD domain of p65 protein sequence. (e) Flag-p65-C, Flag-p65-FL-WT and Flag-p65-mutants were expressed in HEK293T cells. Cells lysates were incubated with GST-HSC70 or GST alone, followed by GST-pull down and immunoblotting analysis as indicated.