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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Differentiation. 2017 Aug 1;96:70–81. doi: 10.1016/j.diff.2017.07.004

Figure 2. Longer differentiation allows the generation of multinucleated myotubes in iPSC-derived myogenic progenitors.

Figure 2

(A) Representative images of immunocytochemistry labeled myogenic cell markers. The plated cells lined up as nascent myotubes after 2 weeks of terminal differentiation and then formed multinucleated myotubes at 6 weeks. Scale bar = 50 μm. (B) Approximately 30–40% of total nuclei expressed Pax7+, MyoD+ and MyoG+ at 2 weeks, but these numbers were significantly reduced at 6 weeks (*p<0.05 vs. 2 weeks) toward myotube maturation. (C) Muscle gene expression on RT-PCR was characterized from mRNA isolation of 0, 2, and 6 weeks differentiated cells.