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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Differentiation. 2017 Aug 1;96:70–81. doi: 10.1016/j.diff.2017.07.004

Table 1.

Primary antibodies used for muscle cell identification.

Antibody Clone Company Dilution
Pax7 Pax7 DSHB (Iowa City, IA, USA) 1:40
Pax3 Pax3 DSHB (Iowa City, IA, USA) 1:40
MyoD 5.8A Vector laboratories (Burlingame, CA, USA) 1:40
MyoG (mouse monoclonal) F5D DSHB 1:40
MyoG (rabbit polyclonal) M-225 Santa Cruz (Dallas, TX, USA) 1:400
MHC MF20 DSHB 1:40
MHC (Alexa Fluor 480 or Alexa Fluor 660 conjugated) MF20 eBioscience (San Diego, CA, USA) 1:500
MYH3 (embryonic MHC) F1.652 DSHB 1:40
MYH8 (fetal/perinatal MHC) N3.36 DSHB 1:40
MYH2 (adult MHC) A4.74 DSHB 1:40
Titin 9D10 DSHB 1:40
Laminin L9393 Sigma-Aldrich 1:100

MyoG, Myogenin; DSHB, Developmental Study Hybridoma Bank.