Figure 6. Transfection with CHOP-overexpression plasmid reverses the effect of 4-PBA on kaempferol-induced HepG2 cell autophagy.

Data are expressed as mean ± SD of at least three independent experiments. (A) The protein expression levels of Atg5, Atg7, Beclin1, LC3B, p62and β-actin were determined by western blot and quantified by densitometry. (B) In the kaempferol-induced HepG2 cells, kaempferol treatment induces severe ER stress, and then promotes the expression level of CHOP, which promotes autophagy to induce HCC cell death.