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. 2017 Jul 18;8(47):82326–82343. doi: 10.18632/oncotarget.19320

Figure 8. hMDMs not only proliferate but also give rise to mammospheres in serum-free media.

Figure 8

hMDMs with the Premo Fucci Cell Cycle Sensor that had engulfed apoptotic MCF-7 cells were sorted by flow cytometry and cultured in chemically defined media. (A) GFP positive cells inside a cell sphere indicate that these cells were in the G2-S phase. Scale bar, 100μm. (B) Cell undergoing mitosis. Two RFP spots inside a cell indicate ongoing cell division. Scale bar, 50μm. (C) Cell sphere with mitosis occurring in the core. Scale bar, 100μm. (D) Expression ofstem cell marker genes in co-cultured macrophages. Marker shown on the left is the 15-1500bp ladder. Stem cell markers examined were Oct4, Sox2 and Nanog. The internal reference gene was β-actin.