Figure 6.

CUR + CQ treated tumor cells arising from nude mice display a faster in vitro growth in correlation with a higher HIF-1α expression. Tumor cells originating from CUR or CUR + CQ treated mice were cultured in vitro for 48 hours. 3 plates containing the same amount of cells were prepared for each tumor and A) observed by optical microscopy or B) analyzed for the expression of HIF-1α by western blot. Tumor cells from CTRL mice were also included. Lamin B was used as loading control. Numbers represent the ratio of specific proteins on the loading control.A representative experiment is shown.