Figure 4. Propoxur augments Nrf2 expression and nuclear translocation and activates ERK1/2 activity.

Both MCF-7 and MDA-MB-231 cells were cultured without or with 0.01, 1 or 100 μM propoxur. (A) Representative blot and electrophoretogram of Nrf2, T-ERK1/2, and p-ERK1/2 proteins, respectively. (B) Representative confocal images of Nrf2 after different dosing of propoxur treatment (magnification, ×400). Following fixation, the cells were stained with a Nrf2 antibody (green) and DAPI (red).