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. 2017 Aug 3;8(50):87136–87150. doi: 10.18632/oncotarget.19896

Figure 10. Effects of NAB2 overexpression in K562 cells on expression of the WT1-target genes VDR, CCND1 and QPRT.

Figure 10

K562 cells were transfected with NAB2, or with empty vector, by electroporation, after which transformed cells were selected for by incubation of cells with puromycin or G418, as described in Material and Methods. After two weeks of selection, mRNA levels were analyzed by qPCR. Shown are levels of VDR mRNA (open bars), CCND1 mRNA (light grey bars), and QPRT mRNA (dark grey bars), in pools of K562 cells overexpressing NAB2, normalized to levels in control cells transfected with empty vector. Mean values, bars ±S.E.M., n = 4. Stars indicate statistical significance (***: p < 0.001; *: p < 0.05; n.s.: not significant).