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. 2017 Aug 3;8(50):87136–87150. doi: 10.18632/oncotarget.19896

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Copyright: © 2017 Nilsson et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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(A, B) 293T/17 cells were transfected with WT1 alone or with NAB2, as indicated. After 48 hours nuclear extracts were prepared, from which WT1 (Santa Cruz, C-19) or NAB2 (Abcam, 1C4) was immunoprecipitated (IP), as indicated. Coprecipitated NAB2 (A) or WT1 (B) was detected by immunoblotting (WB), using anti-WT1 (Santa Cruz, sc-192, clone C19), or anti-NAB2 (Abcam, ab135665). (C) From nuclear extracts of K562 cells, immunoprecipitation was performed with Sepharose only (negative control), with anti-WT1, or with anti-NAB2 (positive control), followed by immunoblotting (WB) with anti-NAB2. The Clean-blot IP detection kit (HRP) (Thermo Scientific) was utilized to eliminate detection-interference from heavy-chain and light-chain IgG-fragments.