Table 5. The primer sequences of IGF1 polymorphisms.
Primer sequence | Position | Restriction enzyme | ||
---|---|---|---|---|
rs972936 | For. | 5′GTTTGAGTCTTTTCAAGCGTTCA3′ | Intron2 | Dra I |
Rev. | 5′ATCTGGGTTAGTCATCTGTGGCA3′ | |||
rs6218 | For. | 5′TCTGTGGAATAAGATACTGGACT3′ | 3′UTR | Dra I |
Rev. | 5′ATCTAACTATGACAGAAAACACG3′ | |||
rs35767 | For. | 5′GAGCCAGAGTAGGATTTCAAGCA3′ | Promoter | Hinf I |
Rev. | 5′CCAGAGCAGACATACCTCTTTCC3′ | |||
rs5742612 | For. | 5′AAAGTATGAGACAGTGCCCTAAA3′ | Promoter | Ava I |
Rev. | 5′GGTAAAGTAGATTGGAAGACAGC3′ |
Note: 3′UTR: 3′ untranslated region.