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. 2017 Sep 30;8(50):88043–88058. doi: 10.18632/oncotarget.21408

Figure 4. Effects of down-, up-regulation of TMEM98 gene on IL-8-induced vascular wall cell function.

Figure 4

Cells after transfected with siRNA or plasmid of TMEM98 co-incubated with BCECF-labeled THP-1 cells for 30min in the presence of IL-8 (A) The number of adherent THP-1 cells in 9 random fields of view was counted. The graphic showed the statistical data of adhesion (B) Cell proliferation was measured by MTT assay (C-F). A7r5 and AoSMC cells were dealt with IL-8 (C and D). A7r5 and AoSMC cells after transfected with siRNA or plasmid of TMEM98 were treatment with IL-8 (E and F). Boyden Chamber assay was conducted to investigate the migration of cells in AoSMC and A7r5 cells (G–J). The graphic H and J showed the statistical data of migration in AoSMC and A7r5. The data were shown as the mean ± SD from 3 independent experiments. Significance was analyzed by using 1-way ANOVA. *p<0.05, **p<0.01, Bars=200μm.