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. Author manuscript; available in PMC: 2018 Sep 19.
Published in final edited form as: Immunity. 2017 Sep 12;47(3):498–509.e6. doi: 10.1016/j.immuni.2017.08.007

Figure 1. SIDT2 localises to late endosomes and interacts with internalised poly(I:C).

Figure 1

(A) MEFs stably expressing SIDT2-mCherry were transfected with markers for (i) early endosomes (EEA1-GFP), (ii) late endosomes (RAB7-GFP) and (iii) lysosomes (LAMP1-YFP), and imaged by confocal microscopy. Co-localization analysis of SIDT2-mCherry with endosomal markers was performed using FIJI software (see main text). Scale bar = 40 μm. (B) DC2.4 cells stably expressing SIDT2-mCherry were transfected with dsDNA-fluorescein or treated with poly(I:C)-fluorescein for 1 h and imaged by confocal microscopy. (C–D) Assessment of SIDT2-mCherry interactions with poly(I:C)-fluorescein and dsDNA-fluorescein in DC2.4 cells via FRET FLIM. ** P < 0.01. Data is plotted as ± SEM and are representative of 3 independent experiments. See also Supplemental videos 1–4.