BMDCs from Sidt2+/+ and
Sidt2−/− mice were treated with
(A) poly(I:C)-rhodamine and (B)
32P-labeled 500bp dsRNA for 60 min at either 4°C or
37°C, and internalisation assessed via flow cytometry or radioactivity
measurement respectively. Results are representative of at least 2 independent
experiments. For panel B, all treatments and measurements were made in
triplicate, and data are plotted as mean ± SEM.
(C–D) BMDCs from
Sidt2+/+ and
Sidt2−/− mice were treated with
poly(I:C) for either 10 or 60 min, stained with J2 anti-dsRNA antibody (red) and
DAPI (blue), and imaged by confocal microscopy. The proportion of each cell
occupied by punctate dsRNA staining was quantified. (E–F)
Sidt2+/+ and
Sidt2−/− BMDCs were treated with
poly(I:C) in association with the cationic polymer DEAE-dextran for 60 min. For
panels C and E, images are representative of at least three independent
experiments. For panels D and F, data are plotted as mean ± SEM and
between 30–150 cells were assessed per time point. *
P < 0.05. Scale bar = 10 μm. See also
Figure S5.