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. Author manuscript; available in PMC: 2017 Dec 15.
Published in final edited form as: Cell. 2016 Dec 15;167(7):1719–1733.e12. doi: 10.1016/j.cell.2016.11.052

Figure 1. Amelioration of Cellular Markers of Aging by Short-Term In Vitro Induction of Oct4, Sox2, Klf4, and c-Myc.

Figure 1

(A) Immunofluorescence of Oct4 and Sox2 in LAKI 4F TTFs. Scale bar, 10 μm.

(B) Immunofluorescence and quantification of γH2AX foci in LAKI 4F TTFs. Scale bar, 10 μm. **** p < 0.0001, according to one-way ANOVA with Bonferroni correction.

(C) qPCR analysis of stress response genes in the p53 pathway, senescence-associated metalloprotease MMP13 and interleukin-6 in LAKI 4F TTFs. **p < 0.01, ***p < 0.001, and ****p < 0.0001 according to one-way ANOVA with Bonferroni correction.

(D and E) Immunofluorescence of H3K9me3 and H4K20me3 in LAKI 4F TTFs. Scale bar, 10 μm. **p < 0.005 and ****p < 0.0001 according to one-way ANOVA with Bonferroni correction.

(F) Immunofluorescence of Lamin A/C and quantification of nuclear abnormality in LAKI 4F TTFs. Arrows indicate blebbing in the nuclear envelope in Oct4 negative cells. Scale bar, 10 μm. **p < 0.005 according to one-way ANOVA with Bonferroni correction.

Data are presented as mean ± SEM. See also Figures S1 and S2.