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. Author manuscript; available in PMC: 2017 Nov 13.
Published in final edited form as: Leukemia. 2017 May 10;31(12):2661–2669. doi: 10.1038/leu.2017.141

Figure 5.

Figure 5

Silencing of KDM6B downregulates MAPK pathway-related genes in MM.

(a) RNA-seq was carried out with MM.1S cells treated with shKDM6B or shLuc. Scatter plot shows differentially expressed transcripts selected based on > 20.3 fold-change with adjusted P < 0.05.

(b,c) Total RNA was isolated from MM.1S and RPMI 8226 cells transduced with shKDM6B or shLuc (b), or sgKDM6B or sgEGFP (c). Expression of 4 MAPK pathway-related genes (RASGRP3, PRKCB, ELK1 and FOS) was then evaluated by quantitative PCR. Expression of each gene relative to control is shown as mean ± s.d. of triplicate measurements. *P <0.05, **P < 0.01, ***P < 0.001 compared with control.