Figure 5.

The dynamic of variant allele frequency (VAF) and acquisition and loss of mutated genes as well as cytogenetic lesions at diagnosis and follow up. VAF and clone size are indicated by the height of the shapes. Cases evolving to s-AML are indicated by the red dashed time line, while follow up without progression to s-AML is indicated by a black dashed time line. Disease course showing (A) gain of gene mutations, (B) loss of a gene mutation, (C) cytogenetic evolution, and (D) no significant change in del(5q) clone size as well as gene mutation (VAF). In (D) both illustrations represent three patients. For the third patient in the first row no cytogenetics was available at s-AML stage. s-AML: secondary acute myeloid leukemia; del(5q): deltion of the long arm of chromosome 5.