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. 2017 Sep 19;8(52):89692–89706. doi: 10.18632/oncotarget.21058

Figure 2. The effects of SYNJ2BP expression on breast cancer cellular EMT phenotype.

Figure 2

(A) Phase-contrast microscopic images of the human breast cancer cell lines MCF-7, T47D, ZR-75-30, MDA-MB-231 (up), and western blot analysis of SYNJ2BP expression in these cells (down). (B) Western blot showing the effects of SYNJ2BP overexpression on the expression levels of E-cadherin, Cytokeratin 18, N-cadherin and Vimientin in MCF-7 and T47D cells. (C) Scratch wound-healing assay assessing the effects of SYNJ2BP overexpression on the motility of MCF-7 and T47D cells. The bar graph shows the fold change of 12 h compare with 0 h wound-healing, 0 h is set to 1. (D) Trans-well migration and invasion assay evaluation the effects of SYNJ2BP overexpression on the cellular motility and invasion ability of MCF-7 and T47D cells. The bar graph shows the number of migrating and invading cells for each category of cells (right).