Skip to main content
. 2017 Sep 19;8(52):89692–89706. doi: 10.18632/oncotarget.21058

Figure 4. SYNJ2BP regulates breast cancer cellular EMT via PI3K/AKT/GSK3β/SNAI1.

Figure 4

(A) Western blots showing that effects of SYNJ2BP overexpression on the expression levels of SNAI1, SLUG, ZEB1. The bar graph showing the relative band intensity of SNAI1, SLUG, ZEB1 expression levels normalized to GAPDH (right). (B) Western blots showing that effects of SYNJ2BP overexpression (left) or knockdown (right) on the expression levels of AKT, pAKT, GSK3β, pGSK3β, SNAI1 and pSNAI1. (C) Western blot showing that effects of SYNJ2BP overexpression with or without LY294002 (24 nM) or U0126 (10 mM) or SB203580 (20 mM) treatment on the expression levels of SNAI1. (D) Western blot showing that effects of SYNJ2BP overexpression with or without LY294002 (24 nM) or LiCl (10 mM) treatment on the expression levels of AKT, pAKT, GSK3β, pGSK3β, SNAI1, E-cadherin and N-cadherin in MCF-7 cells. (E) Western blot analysis showing the reversion of repressed E-cadherin expression in SNAI1-knockdown cells by overexpression of SYNJ2BP. MCF-7 cells without or with SNAI1 knockdown were transfected with SYNJ2BP and the levels of SNAI1, E-cadherin and GAPDH in the cells were analyzed by western blot. RT-PCR analysis (F) and reporter-gene assay (G) showing the level of E-cadherin transcript in the different groups of cells in E.