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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Biochemistry. 2015 Jul 24;54(30):4711–4730. doi: 10.1021/acs.biochem.5b00362

Figure 3. Expression of CvGal2 in Eastern oyster tissues.

Figure 3

(A) mRNA expression of CvGal2 in different tissues of the eastern oyster was assessed by RT-PCR. Relative expression levels relative to actin are shown. (B) CvGal1 and CvGal2 protein in unattached hemocytes, attached hemocytes, or plasma was detected by Western blot. Recombinant CvGal1 (rCvGal1) and CvGal2 (rCvGal2) were included as specificity controls for the antibody, and a membrane exposed to pre-immune IgG is included as a control for IgG specificity. (C) Hemocytes were allowed to attach for 1 hour, and the presence of CvGal2 on cell surface (w/o Triton) or total (with Triton) was revealed by fluorescence microscopy after antibody staining. Images from cells stained with pre-immune IgG (IgG) are included as controls for IgG specificity. Scale bar: 20μm.