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. 2017 Oct 4;8(49):84643–84658. doi: 10.18632/oncotarget.21471

Figure 6. Tumors do not fully regress in response to siL3-TLP and siL2-TLP treatment but retain sensitivity to DISE.

Figure 6

A. Treatment scheme. B. Small animal imaging of 100,000 HeyA8-Nuc-red-LucNeo-Venus-CD95L cells injected i.p. into NGS mice treated with either siScr-TLPs, siL2-TLPs, or siL3-TLPs. Left: Tumor growth over time. Right: Bioluminescence signal in individual mice at the fourth IVIS (22 days after tumor injection) treated as indicated following the treatment protocol outlined in A. ANOVAs were performed for pairwise comparisons of average flux over time between siScr and siL3 treated and siScr and siL3 mice, respectively. C. Immunofluorescence analysis of frozen tumors from three mice of each treatment group in B. Red, nuclei of tumor cells; Green, Venus fluorescence (mouse numbers indicated in top left). D. Change in green fluorescence (RNAi, top 6 panels) and change in red object count (growth, bottom 6 panels) of tumor cells from 3 mice per siScr-TLP and siL3-TLP treatment group after transfection with either siScr or siL3. 1000 cells per well were plated (mouse numbers indicated in top left).