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. 2017 Sep 30;8(49):86681–86692. doi: 10.18632/oncotarget.21407

Figure 2.

Figure 2

(A) Matrigel plug assay. Matrigel mixed with DMEM only, DMEM + mouse VEGF, or culture medium of HuH7-S2, HuH7-core-high, or HuH7-core-low cells was subcutaneously injected into 5-week-old mice. The Matrigel plugs were harvested 7 days later, and their hemoglobin content was evaluated. Data are presented as mean ± SEM. *: p < 0.05; NS = not significant. (B) mRNA expression levels of genes associated with angiogenesis in HuH7-S2, HuH7-core-high, and HuH7-core-low cells were examined through quantitative reverse transcription PCR analysis with GAPDH as the internal control. The value of the HuH7-S2 cells was used as a reference to show the expression level of HuH7-core-high and HuH7-core-low cells. *: p < 0.05 compared to HuH7-S2 cells. (C) Protein expression levels of angiogenic factors in HuH7-S2, HuH7-core-high, and HuH7-core-low cells, examined through Western blot analysis.