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. 2017 Sep 30;8(49):86681–86692. doi: 10.18632/oncotarget.21407

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Copyright: © 2017 Shao et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Validation of the role of VEGF in the proangiogenic activity of HuH7 cells expressing the HCV core protein through (A-D) the tube formation assay and (E-F) the Matrigel plug assay. (A) Images (50×) displaying the influence on tube formation of HUVECs by various HCC cells or control media, with control IgG1 or bevacizumab, an anti-VEGF antibody. (B-D) Quantitative results of the tube formation assay showing the total tube length (B), mean tube area (C), and numbers of branch points (D). Data are presented as mean ± SEM. Low serum growth supplement (LSGS) was the positive control. *: p < 0.05 compared to IgG control. (E) Images displaying the Matrigel plugs harvested 7 days after transplantation. (F) Quantitative analysis of the Matrigel plug assay results through hemoglobin quantification. Matrigel was mixed with the media as noted in the figure with control IgG1 or bevacizumab. Data are presented as mean ± SEM. Human VEGF was the positive control. *: p < 0.05 compared to IgG control.