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. 2017 Sep 26;7(17):4135–4148. doi: 10.7150/thno.20955

Figure 2.

Figure 2

Preparation and characterization of liposome-based pIA complexes. (A) Composition of liposome-based pIA complexes. (B) Formation analysis of liposome-based pIA complexes at various weight ratios (liposome-pIA) by agarose gel electrophoresis. Lane 1: Marker; Lane 2: naked pIA (N, 5 µg); Lane 3-8: liposIA (each sample contains 5 µg of pIA) with progressively increasing weight of liposome solution. (C) Ethidium bromide exclusion assay of liposIA complexes at various weight ratios (n = 3). (D) Size, (E) Polydispersity index (PDI) and (F) Zeta potential of liposIA. Empty carriers as control (Ctr). (G) Expression of the IA fusion protein in HEK-293T cells mediated by naked pIA and liposIA at various weight ratios (each sample contains 5 µg of pIA), assayed 24 h post transfection (n = 3; mean ± SD; *P < 0.05). (H) Serum stability assay of naked pIA and liposIA. Free pIA and liposIA complexes (5 µg) were separately incubated in 50% fetal bovine serum-containing media at 37°C for the indicated durations and degradation of pIA was investigated by 1.0% agarose gel electrophoresis. (I) Quantification of liposIA and naked pIA stability in the presence of serum as compared to PBS controls (n = 3; mean ± SD; **P < 0.01).