Figure 5.

In vitro combinational gene/photothermal therapy of PC-3 cells with siPLK/ZD-GNRs. (a-b) In vitro cytotoxicity of saline, ZD-GNRs, and siPLK/ZD-GNRs with and without 808 nm laser irradiation at power density of 0.5 W/cm² against prostate cancer cells after incubation for 1 day (a) and 2 days (b). (c-d) Confocal microscopy images of PC-3 cells treated with PI and Annexin V (AV) after treatment after incubation for 1 day (c) and 2 days (d) (Blue fluorescence is associated with DAPI; the green fluorescence is expressed by Annexin V-FITC, and the red fluorescence is released from PI). (e-f) Scale bar: 20 μm. Flow cytometry data of PC-3 cells treat with PI and Annexin V (AV) after treatment after incubation for 1 day (e) and 2 days (f).