LH pulse parameters in stress and no-stress female mice. Quantitative summary of the LH responses during the first 90-minute (Pre) or second 90-minute (Post) sampling period in control OVX mice or mice in response to stress (n = 7 mice/group). Mean (± SEM) values for each LH parameter—(A), frequency; (B), interpulse interval; (C), pulse amplitude; (D), mean LH; (E), basal LH—were calculated across the pre and post periods and analyzed by two-way repeated measures ANOVA with Tukey post hoc testing to determine stress effects. Note: Two stress animals did not show pulses during the stress period and were not included in the assessment of LH pulse amplitude. None of the LH parameters differed significantly during the pre period between control and stress groups. *P < 0.05, pre vs post period.