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. Author manuscript; available in PMC: 2017 Nov 20.
Published in final edited form as: Biochemistry. 2016 Apr 18;55(16):2301–2304. doi: 10.1021/acs.biochem.6b00038

Figure 1.

Figure 1

An ssrA-tag at the C-terminus facilitated the degradation of AcrB. (A) AcrB is a trimeric integral membrane protein with 12 transmembrane helices and a large periplasmic domain in each protomer (color-coded) (Created using The PyMOL Molecular Graphics System (Version 1.8 Schrödinger, LLC.) from 2GIF.pdb.32 (B) Anti-AcrB Western blot analyses of membrane extracts obtained from DL41ΔacrB strain containing plasmid- encoded wt-AcrB, AcrB- 11aa ssrA, or AcrB-5aa ssrA. (C) Degradation of S35-Met-AcrB-His6-ssra and S35-Met-AcrB-His6 determined by the pulse-chase experiment. Note the difference in the unit of the time.