Surface-rendered images of the 3-D point-spread functions that are located at increasing distances from the center of a particle. The examples shown here are for radial distances of 0, 125, 250, 500, and 750 Å, going from left to right, respectively. The outer, semitransparent surface is drawn at 1/3 the peak value, and the inner, solid surface is drawn at 2/3 the peak value.