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. 2017 Sep 27;8(54):92454–92469. doi: 10.18632/oncotarget.21384

Figure 4. The knockdown of PLCE1 by siRNA promotes the antitumor effects of miR-34a.

Figure 4

(A) Cell proliferation was measured by MTT at 24, 48, 72, 96, and 120 h in Eca109 cells treated with miR-34a mimic, PLCE1 and siRNA co-transfection, or NC. (B, C) Representative statistics of colony formation in cell lines with above treatment. Original magnification is 200×. (D, E) Cell apoptosis detected by labeled flow cytometry in Eca109 cells at 48 h after transfection. Apoptotic evaluation was performed by calculating the apoptotic percentage of cells. (F, G) Ectopic expression of miR-34a promoted the inhibition of cell migration in PLCE1-siRNA- treated cells. Transwell assays were performed 48h after transfection. The representative numbers of cells across a Matrigel membrane with 8 mm pores. (**P < 0.01). (H) The expression of PLCE1, apoptosis-related proteins, and EMT-related marker proteins in Eca109 cell lines transfected with both miR-34a mimic and PLCE1 siRNA.* P < 0.05, ** P < 0.01, and *** P < 0.001 vs. scramble control (Student's t-test). All results were reproduced by three independent experiments.