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. 2017 Oct 6;8(54):92815–92826. doi: 10.18632/oncotarget.21590

Figure 1. BCAR4 is highly expressed in colon cancer.

Figure 1

(A) Total RNAs were extracted from 20 pairs of human CC samples with Trizol. Expression levels of BCAR4 in tumor and adjacent normal tissues were analyzed by RT-qPCR. Fold changes were normalized to endogenous ACTB. (B) Expression levels of BCAR4 were checked in colon cancer cell lines (HCT8, SW480 and HCT116) and CCD 841 CoN cells by RT-qPCR. Fold changes were normalized to endogenous ACTB. (C) Expression levels of BCAR4 in peritumor and CC samples were examined by Northern blot. 18S was chosen for loading control. Probes were labeled with Biotin. (D) BCAR4 expression in peritumor and CC samples was measured by RNA hybridization in situ with biotin-labeled BCAR4 probes. Scale bars, 100 μm. ***P<0.001 by two-tailed Student's t test. All data presented are shown as means ± SD collected from three independent experiments.