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. 2017 Oct 6;8(54):92815–92826. doi: 10.18632/oncotarget.21590

Figure 6. BCAR4 associates with β-catenin depending on nt 1000-1314.

Figure 6

(A and B) BCAR4 (nt 1000-1314) interacted with β-catenin as shown by RNA pulldown assays. Biotin-labeled BCAR4 or control was added into CC sample lysates. (C) BCAR4 (nt 1000-1314) was essential for its function of preventing β-catenin from degradation. BCAR4 was overexpressed in HCT8 cells. (D) BCAR4 (nt 1000-1314) was essential for activation of Wnt/β-catenin signaling. (E) Deletion of nt 1000-1314 in BCAR4 impaired its potential to promote proliferation of colon cancer cells. HCT8 cells were transfected with BCAR4 or Vector and used for MTT assays. *P<0.05 and **P<0.01 by two-tailed Student's t test. All data presented are shown as means ± SD collected from three independent experiments.