| cell line (human) |
HeLa |
ATCC |
ATCC Cat# CRM-CCL-2; RRID:CVCL_0030
|
Cell line maintained in A. Risinger lab. Authenticated by STR profiling (Genetica). |
| cell line (human) |
Panc-1 |
ATCC |
ATCC Cat# CRL-1469; RRID:CVCL_0480
|
Cell line maintained in A. Risinger lab. Authenticated by STR profiling (Genetica). |
| cell line (human) |
MDA-MB-231 |
ATCC |
ATCC Cat# CRM-HTB-26; RRID:CVCL_0062
|
Cell line maintained in A. Risinger lab. Authenticated by STR profiling (Genetica). |
| cell line (human) |
SK-OV-3 |
ATCC |
ATCC Cat# HTB-77; RRID:CVCL_0532
|
Cell line maintained in A. Risinger lab. Authenticated by STR profiling (Genetica). |
| cell line (human) |
SK-N-BE(2) |
ATCC |
ATCC Cat# CRL-2271; RRID:CVCL_0528
|
Cell line maintained in A. Risinger lab. Authenticated by STR profiling (Genetica). |
| cell line (rat) |
A1A1 |
PMID:8190100 |
|
Cell line maintained in W. Clarke lab |
| antibody |
anti-PGP9.5 (mouse monoclonal) |
Encor Biotechnology |
EnCor Biotechnology Cat# MCA-BH7-AP; RRID:AB_2572395
|
(1:1000) |
| antibody |
anti-collagen type IV (goat polyclonal) |
Southern Biotechnology |
Southern Biotechnology Cat# 1340–01 |
(1:200) |
| antibody |
anti-ATF3 (rabbit polyclonal) |
Santa Cruz Biotechnology |
Santa Cruz Biotechnology Cat# sc-188; RRID:AB_2258513
|
(1:400) |
| antibody |
anti-NeuN (mouse monoclonal) |
Millipore (Chemicon) |
Millipore Cat# MAB377; RRID:AB_2298772
|
(1:250) |
| antibody |
anti-poly(ADP-ribose)10 hr (mouse monoclonal) |
Enzo Life Sciences |
Enzo Life Sciences Cat# ALX-804–220, RRID:AB_2272987
|
(1:300) |
| antibody |
anti-β-tubulin (mouse monoclonal) |
Sigma |
Sigma Cat# T8328; RRID:AB_1844090
|
(1:400) |
| antibody |
anti-mouse Alexa Fluor 488 secondary (donkey polyclonal) |
Jackson ImmunoResearch |
Jackson ImmunoResearch Code# 715-545-150 |
(1:500) |
| antibody |
anti-rabbit Alexa Fluor 594 secondary (donkey polyclonal) |
Jackson ImmunoResearch |
Jackson ImmunoResearch Code# 711-585-152 |
(1:500) |
| antibody |
anti-goat Alexa Fluor 594 secondary (donkey polyclonal) |
Jackson ImmunoResearch |
Jackson ImmunoResearch Code# 705-585-147 |
(1:500) |
| antibody |
anti-mouse IRDye 800CW secondary (goat polyclonal) |
LI-COR Biosciences |
LI-COR Biosciences Cat# 827–08364; RRID:AB_10793856
|
(1:10,000) |
| antibody |
anti-mouse FITC secondary (sheep polyclonal) |
Sigma |
Sigma Cat# F6257; RRID:AB_259692
|
(1:200) |
| other |
DAPI stain |
Sigma (Roche) |
Sigma Cat# 10236276001 |
(0.1 μg/ml) |
| other |
VECTASHIELD HardSet Mounting Medium |
Vector Laboratories |
Vector Laboratories Cat# H-1400; RRID:AB_2336787
|
|
| commercial assay, kit |
NAD/NADH-Glo Assay |
Promega Corporation |
Promega Cat# G9071 |
|
| commercial assay, kit |
Tubulin polymerization assay, OD based - Porcine |
Cytoskeleton, Inc. |
Cytoskeleton Cat# BK006P |
|
