Lycium barbarum polysaccharide protects against neurotoxicity via the Nrf2-HO-1 pathway

Abstract

The incidence of neurodegenerative diseases including Alzheimer's and Parkinson's disease has markedly increased over the past few decades. Oxidative stress is considered to be a common pathophysiological condition resulting in neurotoxicity. Lycium barbarum polysaccharide (LBP) is the major active component of Lycium barbarum L., which exhibit potent antioxidant activity. The current study investigated the neuroprotective effects of LBP in H₂O₂-treated PC12 cells in vitro and in CoCl₂-treated rats in vivo. It was determined that LBP concentration-dependently reversed the H₂O₂-induced increase in reactive oxygen species (ROS) levels, decrease in cell viability, increase in TUNEL-stained cells, increase in caspase-3 and −9 activity and decrease in mitochondrial membrane potential, indicating the amelioration of mitochondrial apoptosis. Furthermore, LBP inhibited the H₂O₂-induced decrease in nuclear factor erythroid 2-related factor 2 (Nrf)2 and heme oxygenase (HO)-1 expression and binding of Nrf2 to the promoters of HO-1. Silencing of Nrf2 and inhibition of HO-1 by zinc protoporphyrin IX (ZnPP) reversed the protective effects of LBP against H₂O₂-resulted neurotoxicity in PC12 cells. In CoCl₂-treated rats, it was demonstrated that LBP decreased brain tissue apoptosis, reduced the time spent by rats finding the platform site, decreased escape latencies and reduced the distance traveled to find the platform. In addition, LBP inhibited the CoCl₂-induced decrease of Nrf2 and HO-1 expression. Administration of ZnPP also suppressed the protective effects of LBP against CoCl₂-resulted neurotoxicity in rats. Thus, the current study indicated that LBP exhibits protective effects against neurotoxicity by upregulating Nrf2/HO-1 signaling. These data may increase understanding regarding the neuroprotective activities of LBP.

Introduction

The incidence of neurodegenerative diseases, such as Alzheimer's disease, has increased dramatically over the past few decades (1,2). Although the molecular mechanism of neurodegenerative diseases is not well understood, oxidative stress is considered to be a common pathophysiological condition that results in neurotoxicity (3,4). The redox system consists of a free radical/reactive oxygen species (ROS)-generating system, oxidants and antioxidant systems (5). Following damage to the antioxidant system or an increase in ROS generation, the balance of the redox status may be disrupted, leading to the development of oxidative stress (5). The central nervous system is particularly susceptible to ROS-induced damage and numerous studies have demonstrated that oxidative stress is closely associated with the development of neurodegenerative diseases. Greilberger et al (6) compared the redox state of the blood between healthy individuals and patients with neurodegenerative diseases. Compared with healthy controls, there were significant elevations in malondialdehyde, protein carbonylation and oxidized albumin levels in patients with neurodegenerative diseases, indicating that there is an association between oxidative stress and the development of neurodegenerative diseases (6). Furthermore, 4-hydroxynonenal-modified proteins have been detected in patients with mild cognitive impairments and throughout the course of Alzheimer's disease (7). In addition, oxidative stress may serve an important role in dopaminergic neurotoxicity (8) and dopamine metabolism-induced alterations in redox status may increase the risk of the onset or progression of Parkinson's disease (9).

The fruits of Lycium barbarum L. (family Solanaceae), more commonly known as Goji berry or wolfberry, have been used in traditional Chinese herbal medicine for thousands of years. Lycium barbarum polysaccharide (LBP) is the major active component found in the fruits. It has been demonstrated that LBP possesses various biological activities, including antioxidant, anti-cancer, anti-inflammatory, anti-aging and immune-regulatory activities (10–15). In particular, it has recently been demonstrated that LBP exhibits potent neuroprotective effects in vivo and in vitro (16–18). Bie et al (16) reported that LBP improved bipolar pulse current-induced microglia cell injury via modulation of autophagy. Furthermore, LBP improved traumatic cognition by reversing the imbalance of apoptosis/regeneration in hippocampal neurons following stress (17). Wang et al (18) determined that LBP prevented focal cerebral ischemic injury by inhibiting neuronal apoptosis in mice. However, the mechanism of the neuroprotective effect of LBP remains unclear.

The present study aimed to investigate the mechanism of the LBP-exerted protective effect against oxidative stress-induced neurotoxicity in vivo and in vitro. The results demonstrated that LBP exhibited neuroprotective activity via activation of nuclear factor erythroid 2-related factor 2 (Nrf) 2/heme oxygenase (HO)-1 signaling.

Materials and methods

Reagents

β-actin (sc-130300) and Nrf2 (sc-722) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) and the HO-1 (RT1270) antibody was purchased from Epitomics; Abcam (Cambridge, MA, USA). MTT and CoCl₂ were purchased from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). Lipofectamine^® 2000, the intracellular superoxide probe dihydroethidium (DHE) and Rhodamine (Rho) 123 were all purchased from Invitrogen; Thermo Fisher Scientific, Inc. (Waltham, MA, USA).

Cell culture and treatment

PC12 cells were purchased from the American Type Culture Collection (Manassas, VA, USA). Cells were cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal calf serum (Gibco; Thermo Fisher Scientific, Inc.), 100 µg/ml penicillin and 100 U/ml streptomycin at 37°C with 5% CO₂ in a humidified incubator. Throughout the experimental treatments, cells were exposed to H₂O₂ in the presence or absence of LBP in serum-free DMEM.

Cell transfection

To test the role of Nrf2 in LBP-exerted protective effect, PC12 cells were transfected with small interfering RNA (siRNA) of Nrf2 and control siRNA before the treatment of H₂O₂ and LBP. Nrf2 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (sc-156128). Transfection was conducted using Lipofectamine 2000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.

Determination of cell viability

Cells were seeded in a 96-well plate at a density of 1×10⁴ cells/well. Cells in the exponential phase were exposed to 0, 50, 100, 200 or 400 µM H₂O₂ and/or 125, 250, 500, 800 or 1,000 µg/ml LBP for 24 h. In some experiments, cells were transfected with Nrf2 siRNA of Nrf2 and control siRNA for 48 h prior to treatment. Cells were then exposed to H₂O₂ and/or LBP in the presence of zinc protoporphyrin IX (ZnPP) (10 µM; Sigma-Aldrich; Merck KGaA), an inhibitor of HO-1. Following the experiments, cell viability was determined by MTT assay. The purple formazan was dissolved in dimethyl sulfoxide. Absorbance was measured at 550 nm and the results are presented as folds of the control cells without H₂O₂ treatment.

Determination of apoptosis

Cells (5×10⁵) were seeded in culture dishes and treated with 400 µM H₂O₂ in the presence or absence of 125, 250 or 500 µg/ml LBP with or without ZnPP for 24 h. In some experiments, cells were transfected with siRNAs for 48 h prior to treatment. Following treatment, cells were fixed with 4% formaldehyde for 15 min at room temperature. Following washing with PBS, the cells were covered with proteinase K solution for 15–20 min. After another PBS wash, the cells were covered with the TUNEL reaction mixture (Roche Applied Science, Penzberg, Germany) and incubated for 1 h in the dark. DAPI counterstaining (10 min at room temperature) was followed by a final PBS wash, and tissue sections were then examined and photographed using confocal microscopy. The average number of fluorescent dots in three images from each treatment group was calculated. Results were expressed as folds of the control.

Determination of caspase activity

Activities of caspase 3 and 9 were determined using a Caspase 3 Activity Assay kit (C1115) and Caspase 9 Activity Assay kit (C1157; Beyotime Institute of Biotechnology, Haimen, China) according to the manufacturer's protocol.

Determination of ROS levels

ROS levels were measured using oxidation-sensitive probes. Cells were incubated with 10 µM DHE for 20 min at 37°C in the dark and were subsequently observed under a confocal fluorescence microscope. In addition, cells were also incubated with 10 µM 2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA, an intracellular hydrogen peroxide probe) (Sigma-Aldrich; Merck KGaA) for 30 min at 37°C in the dark. Subsequently, cells were washed twice with PBS and analyzed using a flow cytometer (BD Accuri™ C6, BD Biosciences, Franklin Lakes, NJ, USA) and BD Accuri C6 software (BD Biosciences). The results were expressed as folds of the control.

Mitochondrial membrane potential

After the treatment of H₂O₂ and LBP, cells were stained with Rhodamine 123 (10 µM) for 20 min at 37°C in the dark. Then the fluorescence was observed under a confocal fluorescence microscope.

Determination of transcription activity

A reporter gene assay was conducted to evaluate the transcriptional activity of Nrf2. PC12 cells (5×10⁴) were seeded into 12-well plates. Subsequently, cells were transfected with 0.2 µg antioxidant response element (ARE)-Luc constructs (Beyotime Institute of Biotechnology, Haimen, China) using Lipofectamine 2000, according to the manufacturer's protocol. pRL-null plasmid (50 ng) encoding Renilla luciferase was included in all samples to ensure transfection efficiency. A total of 48 h after transfection, cells were treated with 400 µM H₂O₂ in the presence or absence of 125, 250 or 500 µg/ml LBP for 24 h. Firefly and Renilla luciferase activities were detected sequentially using the Dual-Glo™ Luciferase assay system (Promega Corporation, Madison, WI, USA).

Chromatin immunoprecipitation (ChIP) assay

Cells were treated with 400 µM H₂O₂ in the presence or absence of 500 µg/ml LBP for 24 h. Subsequently, a ChIP assay was conducted to examine the binding of Nrf2 in HO-1 promoters. Following the experiment, cells were washed, fixed in 1% formaldehyde for 10 min at room temperature and sonicated (on 3 sec and off 10 sec for 10 times at room temperature) to shear chromatin. Nrf2 antibody (dilution 1:50) was added to the cleared lysate and binding was allowed to proceed at 4°C overnight. The complex was eluted and the released DNA was amplified by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) as stated below.

RNA isolation and RT-qPCR

Total RNA was isolated from cells and tissues using Total RNA Isolation kit (Tiangen Biotech, Co., Ltd., Beijing, China) according to the manufacturer's instructions. A total of 0.5 µg RNA was reverse transcribed using the First Strand cDNA synthesis kit (Takara Bio, Inc., Otsu, Japan). qPCR was performed to precisely quantify Nrf2 and HO-1 using SYBR Green reagent (Takara Bio, Inc.). Amplification was performed with an initial step at 94°C for 5 min, followed by 40 cycles of denaturation at 94°C for 30 sec, annealing at 63°C for 30 sec and extension at 72°C for 10 sec. The 2^−ΔΔCq method was used to determine gene expression compared with the endogenous controls (GAPDH) (19). The primers for qPCR analysis were as follows: Nrf2, forward, 5′-ATTGCCTGTAAGTCCTGGTCA-3′, reverse, 5′-ACTGCTCTTTGGACATCATTTCG-3′; HO-1, forward, 5′-TTCCTGGACTGATCCCAATTCTG-3′, reverse, 5′-CTTGGAAGCCACAGAAATGCAG-3′; GAP DH, forward, 5′-GCACCGTCAAGGCTGAGAAC-3′, reverse, 5′-TGGTGAAGACGCCAGTGGA-3′.

Protein extraction and western blotting

Following treatment, cells were lysed and cytoplasmic and nuclear proteins were extracted using NE-PER Nuclear and Cytoplasmic Extraction reagents (Thermo Fisher Scientific, Inc.). Protein concentration was determined using the BCA assay. Lysates containing 20 µg protein were boiled at 100°C for 5 min, separated on a 10% sodium dodecyl sulfate-polyacrylamide gel and transferred to PVDF membranes. Membranes were blocked in 8% skim milk in TBS buffer for 30 min at 37°C. Subsequently, membranes were incubated with the appropriate primary antibodies (Nrf2, HO-1 and β-actin, dilution: 1:500) overnight at 4°C and washed four times with TBST for 10 min each time. Following washing, membranes were further incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-rabbit antibody, dilution: 1:5,000) (32260; Invitrogen, Thermo Fisher Scientific, Inc.) for 30 min at 37°C and then washed another four times. Membranes were visualized using Immobilon Western Chemiluminescent HRP Substrate (Merck KGaA). Bands were captured using a VersaDoc image analysis system (Bio-Rad Laboratories, Inc., Hercules, CA, USA) and quantified with Quantity One software v.4.62 (Bio-Rad Laboratories, Inc.).

Animal treatment

All animal care was conducted in accordance with the guidelines for the Care and Use of Laboratory Animals (15) and the principles presented by School of Medicine, Xi'an Jiaotong University (Shaanxi, China). The present study was approved by the Ethics Committee of Xi'an High-Tech Hospital. All efforts were made to reduce suffering and the number of animals used. A total of 64 adult male Wistar rats weighing 250–300 g (8–10 weeks old) were obtained from the Animal Centre of the School of Medicine, Xi'an Jiaotong University. Rats were housed in a controlled environment, with a 12–12 h light/dark cycle, controlled humidity (50%) and temperature (24°C), and had free access to standard laboratory rat food and water.

Rats were randomly divided into four groups with 16 animals in each group: Control group, CoCl₂ group, CoCl₂+LBP group and CoCl₂+LBP+ZnPP group. Surgery was conducted as previously reported (20–22). Control group: Rats received saline solution. CoCl₂ group: Rats received 50 mM CoCl₂. CoCl₂+LBP group: Rats received 50 mM CoCl₂ and LBP (100 mg/kg) injection. CoCl₂+LBP+ZnPP group: Rats received 50 mM CoCl₂, LBP (100 mg/kg) injection and ZnPP (10 mg/kg) injection. Briefly, rats were placed in a stereotaxic apparatus and received a unilateral lesion by drilling a small hole on the skull in the frontoparietal cortex at Bregma-1.30 mm. Subsequently, a sterile solution of CoCl₂ (50 mM) or an equivalent volume of saline solution was injected in the right hemisphere, 1 mm below the pia level in the cerebral cortex (layers 3–4) with a Hamilton syringe. The CoCl₂ solution was adjusted to reach a physiological osmolarity of 310 mOsm/kg. Following the injection, the lesion site was repaired using the temporal muscle and the attached fascia. During the surgery period, the body temperature of the animals was maintained using a heating pad. Surgical procedures were performed under anesthesia induced with 8% (v/v) sevofluorane inhalation (the dose was selected by preliminary experiments). Concurrent preemptive analgesia was used and a precision vaporiser was used to perform anaesthesia and each animal was put in a separate cage for recovery. Following 2 days recovery, rats in CoCl₂+LBP group and CoCl₂+LBP+ZnPP group received intraperitoneal LBP (100 mg/kg) and rats in CoCl₂+LBP+ZnPP group received ZnPP (10 mg/kg) injection once a day for 10 days. After the treatment, 8 rats in each group were sacrificed using intraperitoneal injection of an overdose of sodium pentobarbital (200 mg/kg; Merck KGaA). Frozen brain sections (5 µm) were cut and then fixed in 4% formaldehyde overnight at room temperature for the determination of apoptosis.

Morris water maze (MWM) test

Immediately after the treatment, an MWM test was conducted to evaluate spatial learning and memory abilities, as previously reported (22,23). The maze was a black circular pool (120 cm in diameter and 45 cm high) divided into four quadrants, with a depth of 30 cm and filled with clear tap water at a temperature of 27±0.5°C. A black platform 10 cm in diameter was located 2 cm below the surface of the water approximately in the middle of one of the four quadrants. Each rat's path in the pool was recorded with Videomex Water Maze Monitoring Software BL-420 (Chengdu Taimeng Technology Co., Ltd., Chengdu, China) and analyzed off-line. Experiments were conducted in a soundproof room and the light source and surrounding environment remained unaltered between each experiment.

During the first 5 days, rats underwent four swimming trials per day, with each trial period limited to 60 sec. In the trials, the time taken to reach the platform, the swimming distance and the speed were recorded. If the rat did not find the platform within the set time, the computer would stop tracking and record the time as 60 sec. If the platform was found within the 60 sec, the rat would be allowed to remain on the platform for 10 sec. Otherwise, the rat would be guided to the platform to remain for 10 sec. On day 6, a probe trial test was conducted. The platform was removed on day 6 for a 60 sec exploration test to record the crossing index to the previous platform site.

Statistical analysis

Statistical analysis was performed using GraphPad Software 5.0 (GraphPad Software, Inc., La Jolla, CA, USA). The results are presented as the mean ± standard error of the mean. The statistical significance of differences between groups was analyzed via one-way analysis of variance followed by a Dunnett's t-test for multiple comparisons. P<0.05 was considered to indicate a significant difference.

Results

LBP inhibits H₂O₂-induced mitochondrial apoptosis in PC12 cells

PC12 cells were incubated with 0, 50, 100, 200 or 400 µM H₂O₂ for 24 h and the results indicated that 100–400 µM H₂O₂ significantly decreased cell viability in a concentration-dependent manner (P<0.05; Fig. 1A). Treatment with 400 µM H₂O₂ decreased cell viability to 20% of the control; thus 400 µM H₂O₂ was used to induce oxidative injury in PC12 cells in subsequent experiments. PC12 cells were incubated with 0, 125, 250, 500, 800 or 1,000 µg/ml LBP for 24 h and the results indicated that LBP significantly decreased cell viability in a concentration-dependent manner at concentrations >500 µg/ml (P<0.05; Fig. 1B). Thus, a concentration of 125–500 µg/ml LBP was selected to examine the effect of LBP on neurotoxicity in PC12 cells in subsequent experiments.

Figure 1.

Effect of LBP on H₂O₂-induced apoptosis. PC12 cells were incubated with (A) 0, 50, 100, 200 or 400 µM H₂O₂ or (B) 0, 125, 250, 500, 800 or 1,000 µg/ml LBP for 24 h. Cell viability was determined by MTT assay and the results are presented as folds of control. (C) PC12 cells were incubated with 400 µM H₂O₂ in the presence or absence of 125, 250 or 500 µg/ml LBP for 24 h. Cell viability was determined by MTT assay and the results are presented as folds of control. (D) Apoptosis was determined by TUNEL staining and the results are presented as folds of control. *P<0.05 vs. control; ^#P<0.05 vs. H₂O₂ treatment. LBP, Lycium barbarum polysaccharide.

LBP dose-dependently reversed the decrease in cell viability induced by H₂O₂ (P<0.05; Fig. 1C). Furthermore, LBP treatment decreased TUNEL-positive cell numbers in H₂O₂-treated cells in a concentration-dependent manner (P<0.05; Fig. 1D). H₂O₂-induced increases in caspase-3 and −9 activity were significantly reversed by LBP in a concentration-dependent manner (P<0.05; Fig. 2A and B). Mitochondrial membrane potential was determined using Rho 123 staining and fluorescence was observed using a confocal microscope. The results demonstrated that LBP markedly inhibited the H₂O₂-induced decrease in Rho 123 fluorescence, indicating a reversal in the decrease of the mitochondrial membrane potential (Fig. 2C). ROS levels were determined by DHE and DCFH-DA staining. The H₂O₂-induced increase in DHE staining was markedly inhibited by LBP (Fig. 2D). Furthermore, LBP significantly suppressed the H₂O₂-induced increase of DCFH-DA-positive cell numbers in a concentration dependent manner (P<0.05; Fig. 2E). These results indicate that LBP concentration-dependently inhibits H₂O₂-induced mitochondrial apoptosis.

Figure 2.

Effect of LBP on the H₂O₂-induced mitochondrial apoptotic pathway. PC12 cells were incubated with 400 µM H₂O₂ in the presence or absence of 125, 250 or 500 µg/ml LBP for 24 h. (A) Caspase-3 and (B) −9 activities were measured using commercial kits and expressed as U/mg protein. (C) PC12 cells were incubated with 400 µM H₂O₂ in the presence or absence of 500 µg/ml LBP for 24 h. Mitochondrial membrane potential was detected using Rho123 staining. Representative images are presented. ROS levels were determined by DHE and DCFH-DA staining. (D) For DHE staining, a representative image is presented. (E) For DCFH-DA staining, fluorescence was analyzed by flow cytometry and the results are presented as folds of control. *P<0.05 vs. control; ^#P<0.05 vs. H₂O₂ treatment. LBP, Lycium barbarum polysaccharide; ROS, reactive oxygen species; DCFH-DA, 7-Dichlorodihydrofluorescein-diacetate; DHE, dihydroethidium; Rho123, Rhodamine 123.

LBP inhibits the H₂O₂-induced decrease of Nrf2/HO-1 signaling in PC12 cells

The potential mechanism responsible for the LBP-induced protective effects against H₂O₂-induced neurotoxicity in PC12 cells was subsequently evaluated. Levels of Nrf2 and HO-1 mRNA and protein were measured. The results demonstrated that the H₂O₂-induced decreases in Nrf2 and HO-1 mRNA expression were significantly reversed by LBP in a concentration-dependent manner (P<0.05; Fig. 3A and B). LBP also markedly reversed the decrease in Nrf2 and HO-1 protein expression induced by H₂O₂ (Fig. 3C). ARE-luciferase activity was significantly reduced by H₂O₂ (P<0.05), however this reduction was reversed by LBP (P<0.05; Fig. 3D). The results of the ChIP assay identified that LBP significantly reversed the H₂O₂-induced decrease of Nrf2 binding to the promoters of HO-1 (P<0.05; Fig. 3E). Cells were transfected with siNrf2 and then exposed to H₂O₂ in the presence or absence of LBP. The results determined that the LBP-induced increase of HO-1 expression in H₂O₂-treated cells was significantly inhibited by Nrf2 silencing induced by siNrf2 (P<0.05; Fig. 3F). These results suggest that Nrf2/HO-1 signaling is involved in the LBP-induced protective effects against neurotoxicity induced by H₂O₂ and indicate that LBP inhibits the H₂O₂-induced decrease of Nrf2/HO-1 signaling in PC12 cells. To determine whether the upregulation of Nrf2/HO-1 signaling is involved in the protective effect of LBP, cells were transfected with siNrf2, then exposed to H₂O₂ in the presence or absence of LBP with or without ZnPP, an inhibitor of HO-1. It was demonstrated that in H₂O₂-treated cells, the LBP-induced decrease in ROS levels (Fig. 4A), increase in cell viability (Fig. 4B) and decrease in cell apoptosis (Fig. 4C) was significantly reversed by siNrf2 and ZnPP (all P<0.05). These results indicate that Nrf2/HO-1 signaling is involved in the LBP-induced protective effects against neurotoxicity induced by H₂O₂ in PC12 cells.

Figure 3.

Effect of LBP on the H₂O₂-induced decrease of Nrf2/HO-1 signaling. PC12 cells were incubated with 400 µM H₂O₂ in the presence or absence of 125, 250 or 500 µg/ml LBP for 24 h. (A and B) mRNA and (C) protein expression of Nrf2 and HO-1 was determined by RT-qPCR and western blotting, respectively. (D) Transcriptional activity of Nrf2 was detected by ARE-luciferase activity. (E) Direct regulation of HO-1 by Nrf2 was determined using a ChIP assay. (F) PC12 cells were transfected with siNrf2 for 48 h and incubated with 400 µM H₂O₂ in the presence or absence of 500 µg/ml LBP for 24 h. HO-1 mRNA expression was determined by RT-qPCR. *P<0.05 vs. control; ^#P<0.05 vs. H₂O₂ treatment; ^P<0.05 vs. LBP treatment. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LBP, Lycium barbarum polysaccharide; Nrf2, nuclear factor erythroid 2-related factor 2; HO-1, heme oxygenase-1; ChIP, Chromatin immunoprecipitation.

Figure 4.

Role of Nrf2/HO-1 signaling in LBP-induced effects on H₂O₂-treated cells. PC12 cells were transfected with siNrf2 for 48 h and subsequently incubated with 400 µM H₂O₂ in the presence or absence of 500 µg/ml LBP with or without ZnPP for 24 h. (A) ROS levels were determined by staining with DCFH-DA. (B) Cell viability was determined by an MTT assay and the results are presented as folds of the control. (C) Apoptosis was determined by TUNEL staining and the results are presented as folds of the control. *P<0.05 vs. control; ^#P<0.05 vs. H₂O₂ treatment; ^P<0.05 vs. LBP treatment. siNrf2, small interfering Nrf2 RNA; Nrf2, nuclear factor erythroid 2-related factor 2; HO-1, heme oxygenase-1; ZnPP, zinc protoporphyrin IX; LBP, Lycium barbarum polysaccharide; DCFH-DA, 7-Dichlorodihydrofluorescein-diacetate; ROS, reactive oxygen species.

Neuroprotective effect of LBP on CoCl₂ in vivo

Subsequently, the neuroprotective effect of LBP against CoCl₂ in vivo was evaluated. Rats were injected with CoCl₂ to construct an animal model of neurotoxicity and subsequently received LBP with or without ZnPP. Following treatment, a MWM test was conducted to evaluate the spatial learning and memory abilities of rats. The CoCl₂-induced decrease of Nrf2 expression was significantly inhibited by LBP (P<0.05) and ZnPP did not significantly affect Nrf2 expression in LBP-treated rats (Fig. 5A). LBP reversed the CoCl₂-induced decrease of HO-1 expression in rat brains (P<0.05; Fig. 5B), however this effect was significantly attenuated by ZnPP (P<0.05; Fig. 5B). A CoCl₂-induced increase in the number of TUNEL-positive cells was inhibited by LBP, indicating a reduction in apoptosis (P<0.05; Fig. 5C). However, ZnPP significantly reversed the anti-apoptotic effect of LBP in CoCl₂-treated rats (P<0.05; Fig. 5C). During the MWM test, rats in CoCl₂ group spent more time finding the platform site, indicated by a significantly lower crossing index compared with control rats (P<0.05; Fig. 5D). Compared with the CoCl₂ group, rats in LBP group spent significantly less time finding the platform site (P<0.05; Fig. 5D), however this decrease in time spent to find the platform site induced by LBP was reversed by ZnPP (P<0.05; Fig. 5D).

Figure 5.

Neuroprotective effect of LBP in rats against CoCl₂. Rats were treated with CoCl₂ and subsequently received intraperitoneal LBP (100 mg/kg) or saline solution with or without ZnPP (10 mg/kg). The expression of (A) Nrf2 and (B) HO-1 mRNA in brain tissue was determined by reverse transcription-quantitative polymerase chain reaction and the results are presented as folds of the control. (C) Apoptosis was determined by TUNEL staining and results were shown as folds of the control. (D) A MWM test was conducted to evaluate spatial learning and memory abilities of rats. Development of spatial memory for platform location during the probe trials following 5 days training during the MWM test. The crossing index represents the average frequency of swims over the platform site in the target quadrant. (E) Average escape latencies. (F) The distance traveled to find the platform. *P<0.05 vs. control; ^#P<0.05 vs. H₂O₂ treatment; ^P<0.05 vs. LBP treatment. Nrf2, nuclear factor erythroid 2-related factor 2; HO-1, heme oxygenase-1; ZnPP, zinc protoporphyrin IX; MWM, morris water maze; LBP, Lycium barbarum polysaccharide.

Rats in the CoCl₂ group exhibited significantly longer escape latencies on test days 3, 4, and 5 than those in the control group (P<0.05; Fig. 5E). Compared with the CoCl₂ group, rats in the LBP group had shorter escape latencies on test days 3, 4, and 5 (P<0.05; Fig. 5E); however this decrease in escape latencies induced by LBP was reversed by ZnPP (P<0.05; Fig. 5E). The distance traveled by the rats in each group to find the platform was also gradually reduced by training and the changing patterns of traveling distance induced by LBP and ZnPP were largely consistent with those for the latency (Fig. 5F). The results demonstrated that LBP may protect against CoCl₂-induced neurotoxicity in vivo and that this may involve the regulation of Nrf2/HO-1 signaling (Fig. 6).

Figure 6.

Mechanism of the neuroprotective effect of LBP. LBP, Lycium barbarum polysaccharide; Nrf2, nuclear factor erythroid 2-related factor 2; HO-1, heme oxygenase-1; ROS, reactive oxygen species.

Discussion

There has been a marked increase in the incidence of neurodegenerative diseases including Alzheimer's and Parkinson's disease over the last few years (1,2). Previous studies have suggested that oxidative stress serves a crucial role in the development of different types of neurotoxicity (24–26). It has been demonstrated that LBP, the major active component of the Lycium barbarum L. fruit, possesses potent antioxidant activity (27–29), thus the current study examined the potential neuroprotective effect of LBP.

In the current study, in vitro experiments were performed on PC12 cells that had undergone H₂O₂-induced neurotoxicity to evaluate the neuroprotective effect of LBP. In addition, CoCl₂-mediated hypoxic neurotoxicity was established in rats to examine the neuroprotective effect of LBP in vivo. It was demonstrated that LBP exhibits potent neuroprotective activity, as indicated by the increase in cell viability and decrease in mitochondrial apoptosis in H₂O₂-treated PC12 cells, as well as the decreased apoptosis in rat brain tissue, decreased time to find the platform site, shorter escape latencies and a shorter distance traveled to find the platform in CoCl₂-treated rats.

There have been few studies assessing the neuroprotective effects of LBP. It has been demonstrated that LBP improves bipolar pulse current-induced microglia cell injury via modulation of autophagy (16). Furthermore, LBP improved traumatic cognition by reversing the imbalance of apoptosis/regeneration that occurs in hippocampal neurons following the induction of stress (17). Wang et al (18) indicated that LBP prevents focal cerebral ischemic injury by inhibiting neuronal apoptosis in mice. Additionally, Rui et al (30) determined that LBP protects rat primary cultured hippocampal neurons against injury induced by oxygen-glucose deprivation and reperfusion. Li et al (31) demonstrated that LBP reduces neuronal damage, disruption of the blood-retinal barrier and oxidative stress in retinal ischemia/reperfusion injury. Based on the aforementioned results and the results of the current study, LBP may exhibit potent neuroprotective activity.

The antioxidant activities of LBP may serve an important role in the protective effects against neurotoxicity, as reflected by the significant reduction in ROS levels that occurs in H₂O₂-treated PC12 cells. Nrf2 is a key transcription factor that determines redox status by regulating numerous antioxidant enzymes (32,33). HO-1 is an important target gene of Nrf2; HO-1 exhibits antioxidant, anti-apoptotic and anti-inflammatory properties via bilirubin/biliverdin and carbon monoxide, the products of HO-1-catalyzed heme degradation (34). It has been demonstrated that disruption to the Nrf2-ARE pathway contributes to the development of neurotoxicity and neurodegenerative diseases (35–37). Activation of Nrf2/HO-1 signaling may be an important method of protecting against neurotoxicity (38–40). It has been indicated that dietary LBP stimulates the Nrf2/ARE pathway and ameliorates insulin resistance induced by a high-fat diet (41). Furthermore, activation of the Nrf2/HO-1 antioxidant pathway may contribute to the protective effects of LBP in the rodent retina following ischemia-reperfusion-induced damage (42). In the present study, the potential role of Nrf2/HO-1 signaling in the neuroprotective effects of LBP was examined. LBP inhibited the reduction of Nrf2/HO-1 signaling in H₂O₂-treated PC12 cells and CoCl₂-treated rats. Nrf2 silencing significantly inhibited the protective effects of LBP in H₂O₂-treated PC12 cells. Furthermore, administration of ZnPP suppressed the protective effects of LBP in H₂O₂-treated PC12 cells and CoCl₂-treated rats. These results demonstrate that the Nrf2/HO-1 pathway may, at least partly, be responsible for the neuroprotective effects of LBP.

In conclusion, the current study demonstrated that LBP exhibits protective effects against neurotoxicity via upregulation of Nrf2/HO-1 signaling. Enhancement of Nrf2/HO-1 signaling contributed to an improvement of oxidative stress and the amelioration of apoptosis. These data may improve understanding of the neuroprotective activities of LBP.