Lactones from the Sponge-Derived Fungus Talaromyces rugulosus

Abstract

The marine-derived fungus Talaromyces rugulosus isolated from the Mediterranean sponge Axinella cannabina and cultured on solid rice medium yielded seventeen lactone derivatives including five butenolides (1–5), seven (3S)-resorcylide derivatives (6–12), two butenolide-resorcylide dimers (13 and 14), and three dihydroisocoumarins (15–17). Among them, fourteen compounds (1–3, 6–16) are new natural products. The structures of the isolated compounds were elucidated by 1D and 2D NMR (Nuclear Magnetic Resonance) spectroscopy as well as by ESI-HRMS (ElectroSpray Ionization-High Resolution Mass Spectrometry). TDDFT-ECD (Time-Dependent Density Functional Theory-Electronic Circular Dichroism) calculations were performed to determine the absolute configurations of chiral compounds. The butenolide-resorcylide dimers talarodilactones A and B (13 and 14) exhibited potent cytotoxicity against the L5178Y murine lymphoma cell line with IC₅₀ values of 3.9 and 1.3 µM, respectively.

1. Introduction

Marine-derived fungi are prolific sources for bioactive secondary metabolites, such as the famous antibiotic cephalosporin C obtained from the fungus Acremonium chrysogenum [1], or halimide derived from an algiculous fungus, which gave rise to the semisynthetic metabolite plinabulin that is now in Phase II clinical studies for its anticancer potential against either solid tumours or lymphomas [2,3].

During our ongoing search for new bioactive secondary metabolites from fungal origin [4,5,6,7], we investigated Talaromyces rugulosus, which was isolated from the Mediterranean sponge Axinella cannabina. The fungal genus Talaromyces is known to produce diverse bioactive secondary metabolites including tetraene lactones [8], polyketides [9,10], alkaloids and peptides with some of them exhibiting antibiotic, cytotoxic and anti-HBV (Hepatitis B Virus) activities [11]. Only a few research papers are devoted to the chemical constituents of Talaromyces rugulosus. Yilmaz et al. reported the isolation of skyrin, endocrocin, emodin, prugosins A-C and a bis-anthraquinoid pigment, rugulosin A, which showed antibacterial activity against Staphylococcus aureus [12]. This encouraged us to investigate the natural products of the sponge-derived strain of T. rugulosus.

The EtOAc (ethyl acetate) extract of the fungus when fermented on solid rice medium yielded three new butenolides (1–3), seven new resorcylide derivatives (6–12), two new butenolide-resorcylide dimers (13 and 14) and two new dihydroisocoumarins (15 and 16) as well as three known analogues (4, 5 and 17) (Figure 1). The structure elucidation including the absolute configuration assignment and biological activities of the isolated compounds are discussed in this report.

Figure 1.

Structures of the compounds isolated from Talaromyces rugulosus.

2. Results

2.1. Structure Elucidation

Compound 1 was isolated as a brown amorphous solid. It has the molecular formula C₁₇H₂₀O₅ as determined by ESI-HRMS (ElectroSpray Ionization-High Resolution Mass Spectrometry) corresponding to eight degrees of unsaturation. Its UV absorbances at λ_max 207, 218 and 288 nm were similar to those of the co-isolated known butenolide derivative lactone acid (4) [13]. The NMR (Nuclear Magnetic Resonance) data of compound 1 (Table 1) were likewise comparable to those of 4 and included typical signals for a mono-substituted benzene ring at δ_H 7.75 (2H, d, J = 7.5 Hz, H-9 and H-13), 7.44 (2H, t, J = 7.5 Hz, H-10 and H-12) and 7.35 (1H, t, J = 7.5 Hz, H-11), an oxygenated methine proton at δ_H 5.52 (H-4), as well as two methylene groups at δ_H 2.45, 1.75 (H_ab-5) and 2.44 (H₂-6). The COSY (Correlation SpectroscopY) correlations between H-4/H₂-5 and H₂-5/H₂-6 together with the HMBC (Heteronuclear Multiple Bond Correlation) correlations from H-4 to C-1 (δ_C 171.0), C-2 (δ_C 139.5), C-3 (δ_C 130.6) and C-8 (δ_C 131.9) and from H_ab-5 and H₂-6 to C-7 (δ_C 174.6) indicated that compound 1 shared the same core structure as 4. In addition, signals of a methyl at δ_H 0.94 (Me-4′) and three methylene groups at δ_H 1.38 (H₂-3′), 1.60 (H₂-2′), 4.08 (H-1′a) and 4.04 (H-1′b) were observed. The COSY correlations between Me-4′/H₂-3′/H₂-2′/H_ab-1′ and the HMBC correlation from H_ab-1′ to C-7 confirmed a n-butyl side chain to be attached to C-7 through an ester bond. Thus, the planar structure of 1 was elucidated as an unsaturated γ-lactone with propanoic acid n-butyl ester side chain. The absolute configuration of 1 was elucidated by comparing its experimental ECD spectrum with that of WF-3681 methyl ester [14], which can be considered a close analogue of 1. Based on the good overall agreement, 1 has (S) absolute configuration.

Table 1.

¹H and ¹³C NMR (Nuclear Magnetic Resonance) Data for compounds 1–3.

No.	1 a		2 a		3 a
	δC, Type	δH (J in Hz)	δC, Type	δH (J in Hz)	δC, Type	δH (J in Hz)
1	171.0, C		168.3, C		170.0, C
2	139.5, C		142.7, C		141.6, C
3	130.6, C		124.6, C		128.7, C
4	79.3, CH	5.52, dd (8.1, 2.2)	109.8, C		86.8, C
5	30.5, CH2	2.45, m	34.6, CH2	2.54, ddd (14.5, 7.9, 6.5)	30.0, CH2	2.68, ddd (15.2, 8.7, 6.4)
		1.75, m		2.18, ddd (14.5, 7.9, 6.5)		2.48, ddd (15.2, 8.7, 6.4)
6	30.0, CH2	2.44, m	29.4, CH2	2.42, ddd (16.0, 7.9, 6.5)	29.1, CH2	2.25, ddd (15.8, 8.7, 6.4)
				2.31, ddd (16.0, 7.9, 6.5)		2.15, ddd (15.8, 8.7, 6.4)
7	174.6, C		174.5, C		174.2, C
8	131.9, C		131.3, C		131.3, C
9, 13	128.6, CH	7.75, d (7.5)	128.6, CH	7.93, d (7.5)	128.6, CH	7.76, d (7.4)
10, 12	129.7, CH	7.44, t (7.5)	129.8, CH	7.45, t (7.5)	129.7, CH	7.42, t (7.4)
11	129.7, CH	7.35, t (7.5)	129.8, CH	7.36, t (7.5)	129.9, CH	7.35, t (7.4)
14					171.9, C
1′	65.6, CH2	4.08, dt (10.8, 6.6)	65.6, CH2	4.02, dt (10.8, 6.7)	65.7, CH2	4.00, dt (10.8, 6.7)
		4.04, dt (10.8, 6.6)		3.97, dt (10.8, 6.7)		3.94, dt (10.8, 6.7)
2′	31.7, CH2	1.60, m	31.7, CH2	1.56, m	31.6, CH2	1.54, m
3′	20.2, CH2	1.38, m	20.2, CH2	1.36, m	20.1, CH2	1.34, m
4′	14.0, CH3	0.94, t (7.3)	14.0, CH3	0.93, t (7.3)	14.0, CH3	0.91, t (7.3)
OMe-4			50.7, CH3	3.18, s

^a Recorded at 300 MHz (¹H) and 75 MHz (¹³C) in CD₃OD.

The UV (UltraViolet) spectrum of compound 2 was similar to that of 1. The ESI-HRMS data revealed the molecular formula C₁₈H₂₂O₆, suggesting the presence of an additional methoxy group in 2 compared to 1, which was observed at δ_H 3.18 and δ_C 50.7, respectively. The disappearance of the oxygenated methine proton along with the HMBC correlation from the protons of the methoxy group to C-4 (δ_C 109.8) indicated this additional methoxy group to be located at the C-4 position. The remaining structure of 2 was elucidated to be identical to that of 1 as indicated by detailed analysis of the 2D NMR spectra of 2. Thus, compound 2 was identified as 4-methoxylactone acid n-butyl ester. The ECD spectrum of 2 was rather weak and noisy, but similar to that of chaetobutenolide C [14], which can be used for ECD correlation and indicated a non-racemic mixture of (R) and (S) isomers with a slight excess of the (R) enantiomer.

The molecular formula of 3 was deduced as C₁₈H₂₀O₇ from ESI-HRMS data. The UV and NMR data (Table 1) of 3 resembled those of the co-isolated lactone diacid (5) [13], except for the observation of an additional n-butyl chain as described for 1. In the HMBC spectrum of 3, H₂-5 (δ_H 2.68 and 2.48), H₂-6 (δ_H 2.25 and 2.15) and H₂-1′ (δ_H 4.00 and 3.94) exhibited correlations to C-7 (δ_C 174.2) while only H₂-5 showed correlation to C-14 (δ_C 171.9), indicating the O-n-butyl group to be attached to C-7. Thus, compound 3 was elucidated as lactone diacid 7-O-n-butyl ester. Compound 3 decomposed before ECD measurements. Its absolute configuration can be tentatively assigned upon biosynthetic considerations as (R) similarly to 5.

To elucidate the absolute configuration of the known natural product 5, the solution TDDFT-ECD (Time-Dependent Density Functional Theory-Electronic Circular Dichroism) protocol was applied on the arbitrarily chosen (R) enantiomer [15,16]. Merck Molecular Force Field (MMFF) conformational search with an implicit solvent model for CHCl₃ resulted in 149 conformers in a 21 kJ/mol energy window. DFT reoptimization of these yielded 9, 12 and 12 low-energy (≥2%) conformers at the B3LYP/6-31G(d) in vacuo, the B97D/TZVP PCM (Polarizable Continuum Model)/MeCN and the CAM-B3LYP/TZVP PCM/MeCN levels, respectively. Boltzmann-averaged ECD spectra of (R)-5 resembled the experimental ECD spectrum allowing elucidation of the absolute configuration as (R) (Figure 2).

Figure 2.

(a) structures and populations of the low-energy B3LYP/6-31G(d) in vacuo conformers (≥2%) of (R)-5; (b) experimental ECD spectrum of 5 in MeCN compared with the Boltzmann-weighted BH&HLYP/TZVP ECD spectrum of (R)-5 computed for the B3LYP/6-31G(d) in vacuo conformers. Bars represent the rotational strength values of the lowest-energy conformer.

The NMR data of compounds 6–10 were found to be identical to those of (3R)-cis-resorcylide, (3R,7R)-7-hydroxyresorcylide, (3R,7S)-7-hydroxyresorcylide, (3R,7R)-7-methoxyresorcylide and (3R,7S)-7-methoxyresorcylide, respectively (Table 2 and Table 3). These resorcylides were first reported by Barrow et al. and the absolute configuration at C-3 was suggested to be S in consideration of their biosynthesis [17]. Later, it was revised to (3R) by chemical derivatization, ECD calculation and X-ray diffraction analysis [18,19,20]. However, the resorcylides isolated in this study exhibited mirror-imaged CD (Circular dichroism) curves compared to those of the corresponding compounds found in the literature, indicating that they should be enantiomers of the previously reported resorcylides derivatives and possess 3S configuration. The above-mentioned TDDFT-ECD protocol was carried out on the arbitrarily chosen (R) enantiomer of 6 yielding 33 MMFF conformers, and 7 and 12 low-energy conformers at the B3LYP/6-31G(d) and the B97D/TZVP levels. Boltzmann averaged ECD spectra computed for both sets of conformers gave mirror-image agreement with the experimental spectrum, allowing elucidation of the absolute configuration as (S) in line with the literature (Figure 3).

Table 2.

¹H and ¹³C NMR Data for compounds 6–8.

No.	6 a		7 b		8 b
	δC, Type	δH (J in Hz)	δC, Type c	δH (J in Hz)	δC, Type c	δH (J in Hz)
1	172.9, C		n.d. d		n.d. d
3	76.9, CH	5.03, m	75.5, CH	4.95, m	73.1, CH	5.19, m
4	32.2, CH2	1.87, m	33.1, CH2	1.91, m	32.9, CH2	1.68, m
		1.65, m		1.75, m
5	26.4, CH2	1.68, m	21.5, CH2	1.70, m	18.9, CH2	1.58, m
				1.43, m
6	27.6, CH2	2.52, m	36.5, CH2	1.58, m	36.8, CH2	1.81, m
		2.21, m				1.50, m
7	139.9, CH	5.81, ddd (11.8, 10.0, 5.7)	66.2, CH	4.39, m	66.9, CH	4.36, m
8	132.8, CH	6.51, br d (11.8)	53.5, CH2	3.03, dd (13.0, 3.1)	51.3, CH2	2.94, dd (15.4, 10.1)
				2.42, dd (13.0, 10.3)		2.61, dd (15.4, 1.6)
9	204.7, C		206.3, C		205.2, C
10	51.5, CH2	4.58, d (18.4)	50.2, CH2	4.84, d (18.4)	52.3, CH2	4.59, d (18.5)
		3.65, d (18.4)		3.75, d (18.4)		3.90, d (18.5)
11	140.1, C		140.0, C		140.2, C
12	113.9, CH	6.16, d (2.5)	113.5, CH	6.23, d (2.5)	113.2, CH	6.23, d (2.5)
13	164.1, C		162.7, C		162.9, C
14	103.0, CH	6.26, d (2.5)	102.2, CH	6.31, d (2.5)	102.3, CH	6.32, d (2.5)
15	167.1, C		165.5, C		166.4, C
16	106.2, C		106.3, C		105.6, C
17	21.5, CH3	1.28, d (6.2)	20.9, CH3	1.31, d (6.1)	18.2, CH3	1.29, d (6.4)
7-OMe

^a Recorded at 600 MHz (¹H) and 150 MHz (¹³C) in CD₃OD. ^b Recorded at 600 MHz (¹H) and 150 MHz (¹³C) in CD₃COCD₃. ^c Data extracted from HSQC (Heteronuclear Single Quantum Correlation) and HMBC. ^d n.d. = not detected.

Table 3.

¹H and ¹³C NMR Data for compounds 9 and 10.

No.	9 a		10 a
	δC, Type	δH (J in Hz)	δC, Type	δH (J in Hz)
1	172.5, C		172.3, C
3	76.1, CH	4.93, m	73.8, CH	5.19, m
4	33.8, CH2	1.82, m	33.5, CH2	1.63, m
		1.75, m
5	21.7, CH2	1.68, m	19.0, CH2	1.58, m
		1.40, m
6	34.4, CH2	1.69, m	34.0, CH2	1.87, m
		1.50, m		1.49, m
7	77.1, CH	3.95, m	78.2, CH	3.90, m
8	49.6, CH2	3.10, dd (13.3, 2.8)	47.4, CH2	2.89, dd (15.7, 9.7)
		2.40, dd (13.3, 10.2)		2.71, dd (15.7, 1.0)
9	208.8, C		208.1, C
10	51.0, CH2	4.68, d (18.7)	53.2, CH2	4.63, d (18.8)
		3.80, d (18.7)		3.84, d (18.8)
11	139.6, C		139.9, C
12	113.9, CH	6.14, d (2.5)	113.7, CH	6.13, d (2.5)
13	163.7, C		163.9, C
14	102.9, CH	6.25, d (2.5)	103.0, CH	6.25, d (2.5)
15	166.0, C		166.9, C
16	107.0, C		106.4, C
17	21.1, CH3	1.32, d (6.1)	18.5, CH3	1.29, d (6.4)
7-OMe	56.7, CH3	3.42, s	56.5, CH3	3.34, s

^a Recorded at 600 MHz (¹H) and 150 MHz (¹³C) in CD₃OD.

Figure 3.

(a) structures and populations of the low-energy B3LYP/6-31G(d) in vacuo conformers (≥2%) of (R)-6; (b) experimental ECD spectrum of 6 in MeCN compared with the Boltzmann-weighted PBE0/TZVP ECD spectrum of (R)-6 computed for the B3LYP/6-31G(d) in vacuo conformers. Bars represent the rotational strength values of the lowest-energy conformer.

Similar ECD pattern was observed in the experimental ECD spectrum of 9, and good agreement of the experimental and TDDFT-ECD spectra allowed determining the absolute configuration as (3S,7S)-9 (Figure S92). The calculations also confirmed that the stereochemistry of the C-3 lactone carbon has dominant contribution to the ECD spectra. Thus, compounds 6–10 are determined as (3S)-cis-resorcylide, (3S,7S)-7-hydroxyresorcylide, (3S,7R)-7-hydroxyresorcylide, (3S,7S)-7-methoxy-resorcylide and (3S,7R)-7-methoxyresorcylide, respectively.

Based on ESI-HRMS data, the molecular formula of compound 11 was determined to be C₂₀H₂₈O₆, containing an additional C₄H₈ unit when compared to 7. Comparison of the ¹H and ¹³C NMR data of 7 and 11 (Table 4) revealed the existence of an additional n-butyl group in 11, which is further confirmed by the COSY correlations between Me-4′ (δ_H 0.95)/H₂-3′ (δ_H 1.42), H₂-3′/H₂-2′ (δ_H 1.56), H₂-2′/H_a-1′ (δ_H 3.63) and H₂-2′/H_b-1′ (δ_H 3.51). Examination of the 2D NMR spectra of 11 indicated it to share the same resorcylide ring structure as found in compounds 7–10. Key HMBC correlations from H_ab-1′ to C-7 (δ_C 75.4) and from H-7 (δ_H 4.04) to C-1′ (δ_C 69.7) confirmed the linkage between C-7 and the additional n-butyl group via an ether bond in 11. Thus, the planar structure of 11 was elucidated as 7-O-n-butylresorcylide. Compound 12 shared the same gross structure as 11 as confirmed by 2D NMR and ESI-HRMS data. The structural difference between 11 and 12 is confined to the configuration at C-7 as already found for 7 and 8, and for 9 and 10. Comparison of the NMR data of 11 and 12, especially of the coupling constants between H-7 and H_ab-8 and of the chemical shifts of C-3, H-3 and C-17 (Table 5), indicated that 11 shared the same relative configuration as 7 and 9, whereas 12 shared the same relative configuration as 8 and 10. Moreover, the CD data of 11 were comparable to those of 7 and 9, whereas the ECD data of 12 resembled those of 8 and 10. The above findings revealed compounds 11 and 12 to be (3S,7S)-7-O-n-butylresorcylide and (3S,7R)-7-O-n-butylresorcylide, respectively.

Table 4.

¹H and ¹³C NMR Data for compounds 11 and 12.

No.	11 a		12 b
	δC, Type	δH (J in Hz)	δC, Type	δH (J in Hz)
1	172.5, C		172.3, C
3	76.2, CH	4.94, m	73.9, CH	5.19, m
4	33.8, CH2	1.80, m	33.6, CH2	1.64, m
		1.75, m
5	21.9, CH2	1.69, m	19.1, CH2	1.58, m
		1.40, m
6	34.6, CH2	1.66, m	34.3, CH2	1.84, m
		1.50, m		1.51, m
7	75.4, CH	4.04, m	76.5, CH	3.98, m
8	50.3, CH2	3.09, dd (13.2, 3.0)	48.2, CH2	2.90, dd (15.6, 9.8)
		2.40, dd (13.2, 10.3)		2.69, dd (15.6, 1.0)
9	208.9, C		208.3, C
10	50.9, CH2	4.69, d (18.7)	53.1, CH2	4.61, d (18.8)
		3.78, d (18.7)		3.84, d (18.8)
11	139.6, C		139.9, C
12	114.0, CH	6.13, d (2.5)	113.8, CH	6.12, d (2.5)
13	163.7, C		163.9, C
14	102.9, CH	6.25, d (2.5)	103.1, CH	6.25, d (2.5)
15	166.0, C		166.8, C
16	107.0, C		106.5, C
17	21.1, CH3	1.31, d (6.1)	18.6, CH3	1.29, d (6.4)
1′	69.7, CH2	3.63, dt (9.2, 6.4)	69.5, CH2	3.51, dt (9.3, 6.5)
		3.51, dt (9.2, 6.4)		3.45, dt (9.3, 6.5)
2′	33.2, CH2	1.56, m	33.2, CH2	1.53, m
3′	20.4, CH2	1.42, m	20.4, CH2	1.38, m
4′	14.3, CH3	0.95, t (7.3)	14.2, CH3	0.93, t (7.3)

^a Recorded at 300 MHz (¹H) and 75 MHz (¹³C) in CD₃OD. ^b Recorded at 500 MHz (¹H) and 125 MHz (¹³C) in CD₃OD.

Table 5.

Key differences of NMR Data for compounds 7–14.

Compounds	7	9	11	13	8	10	12	14
3J7,8a (Hz)	3.1	2.8	3.0	3.1	10.1	9.6	9.8	10.4
3J7,8b (Hz)	10.3	10.2	10.3	10.4	1.6	1.0	1.0	- a
C-3	75.5	76.1	76.2	76.1	73.1	73.8	73.9	74.0
H-3	4.95	4.93	4.94	4.93	5.19	5.19	5.19	5.13
C-17	20.9	21.1	21.1	20.9	18.2	18.5	18.6	18.9

^a Signals overlapped.

Compound 13 was isolated as a brown amorphous solid. Its molecular formula was established as C₃₁H₃₂O₁₂ from ESI-HRMS data. Interestingly, the ¹H and ¹³C NMR spectra of 13 (Table 6) showed two sets of signals corresponding to the macrolide (3R,7R)-7-hydroxyresorcylide (7) and the butenolide lactone diacid (5), which were co-isolated in the present study. For example, the two meta-coupling aromatic protons at δ_H 6.26 (d, J = 2.5 Hz, H-14) and 6.14 (d, J = 2.5 Hz, H-12), the two oxygenated methine protons at δ_H 5.54 (m, H-7) and 4.93 (m, H-3), the isolated methylene protons at δ_H 4.67 (d, J = 18.8 Hz, H_a-10) and 3.78 (d, J = 18.8 Hz, H_b-10) and the doublet methyl at δ_H 1.28 (d, J = 6.2 Hz, Me-17) represented characteristic signals for 7-hydroxyresorcylide. On the other side, signals of the mono-substituted benzene ring at δ_H 7.73 (d, J = 7.7 Hz, H-9′ and H-13′), 7.44 (2H, t, J = 7.7 Hz, H-10′ and H-12′) and 7.37 (1H, t, J = 7.7 Hz, H-11′) along with those of two methylene groups at δ_H 2.77, 2.59 (H_ab-5′) and 2.29, 2.22 (H_ab-6′) indicated the butenolide lactone diacid (5).

Table 6.

¹H and ¹³C NMR Data for compounds 13 and 14.

No.	13 a		14 a
	δC, Type	δH (J in Hz)	δC, Type	δH (J in Hz)
1	172.4, C		172.2, C
3	76.1, CH	4.93, m	74.0, CH	5.13, m
4	33.4, CH2	1.75, m	33.8, CH2	1.66, m
		1.61, m
5	21.2, CH2	1.39, m	19.0, CH2	1.52, m
		1.31, m
6	33.2, CH2	1.57, m	32.8, CH2	1.84, m
				1.56, m
7	72.9, CH	5.54, m	74.2, CH	5.53, m
8	49.2, CH2	2.94, dd (13.3, 3.1)	46.9, CH2	3.07, dd (15.8, 10.4)
		2.58, dd (13.3, 10.4)		2.49, d (15.8)
9	206.6, C		206.1, C
10	50.8, CH2	4.67, d (18.8)	52.8, CH2	4.52, d (18.8)
		3.78, d (18.8)		3.73, d (18.8)
11	139.3, C		139.6, C
12	114.1, CH	6.14, d (2.4)	113.8, CH	6.07, d (2.5)
13	163.8, C		163.9, C
14	103.0, CH	6.26, d (2.4)	103.0, CH	6.24, d (2.5)
15	166.2, C		166.8, C
16	106.7, C		106.2, C
17	20.9, CH3	1.28, d (6.2)	18.9, CH3	1.27, d (6.4)
1′	169.8, C		169.7, C
2′	141.9, C		141.8, C
3′	128.1, C		128.6, C
4′	86.6, C		86.6, CH
5′	30.1, CH2	2.77, ddd (15.2, 8.6, 6.4)	30.0, CH2	2.72, ddd (15.3, 8.7, 6.5)
		2.59, ddd (15.2, 8.6, 6.4)		2.49, ddd (15.3, 8.7, 6.5)
6′	28.7, CH2	2.29, ddd (16.0, 8.6, 6.4)	28.7, CH2	2.23, ddd (15.9, 8.7, 6.5)
		2.22, ddd (16.0, 8.6, 6.4)		2.14, ddd (15.9, 8.7, 6.5)
7′	174.4, C		175.9, C
8′	131.0, C		131.0, C
9′, 13′	128.6, CH	7.73, d (7.7)	128.5, CH	7.70, d (7.4)
10′, 12′	129.9, CH	7.44, t (7.7)	129.9, CH	7.44, t (7.4)
11′	130.1, CH	7.37, t (7.7)	130.1, CH	7.37, t (7.4)
14′	169.9, C		169.5, C
OMe	52.3, C	3.58, s

^a Recorded at 700 MHz (¹H) and 175 MHz (¹³C) in CD₃OD.

Detailed analysis of the 2D NMR spectra of 13 established the two subunits of resorcylide and butenolide as shown. The HMBC correlations from H-7 and H_ab-5′ to C-14′ (δ_C 169.9) indicated the connection of these two subunits through the C(7)-O-C(14′) ester bond. In addition, the attachment of a methoxy group (δ_H 3.58 and δ_C 52.3) at C-7′ was deduced by the HMBC correlation from the protons of the methoxy group to C-7′ (δ_C 174.4). Thus, the structure of compound 13 was elucidated as shown, representing a butenolide-resorcylide dimer, for which the name talarodilactone A is proposed. Comparison of its NMR data with those of other resorcylide derivatives (Table 5) indicated that compound 13 shared the same relative configuration as 7, 9 and 11 regarding the macrolactone part. The ECD spectrum of 13 was found to be similar to those of resorcylide monomers, which suggested that the ECD spectrum of 13 is mainly governed by the C-3 chiral center of the resorcylide moiety and thus the absolute configuration of this chiral center could be unambiguously elucidated as (3S). On the basis of the (3S*,7S*) relative configuration determined by NMR experiments, the absolute configuration of the RAL subunit of 13 was determined as (3S,7S). Considering the shape of the ECD spectrum of 13, it more resembles those of 7 and 9 than those of 8 and 10 supporting the assignment of C-7 as (S). The absolute configuration of the butenolide part can be tentatively assiged as (R) on the basis of biosynthetic considerations.

The molecular formula of compound 14 was determined to be C₃₀H₃₀O₁₂, suggesting the loss of one methoxy group compared to 13, which was confirmed by the disappearance of the respective signal in the NMR spectra of 14. Furthermore, comparison of the NMR data of 14 with those of the other resorcylide derivatives isolated in this study (Table 5) revealed that 14 shared the same relative configuration as 8, 10 and 12 at C-3 and C-7. Similarly to 13, the (3S) absolute configuration of 14 can be unambiguously deduced from the ECD spectrum, while that of C-7 can be determined as (R) by utilizing the experimental NMR data. The C-4′ chiral center can be only tentatively assigned as (R) on the basis of biosynthetic considerations.

Compound 15 was isolated as a brown amorphous solid, exhibiting UV absorbance maxima at 220, 258 and 334 nm. Its molecular formula was found to be C₁₂H₁₂O₆ as confirmed by ESI-HRMS data, indicating seven degrees of unsaturation. The ¹H NMR spectrum of 15 (Table 7) showed two ortho-coupling protons at δ_H 7.01 (d, J = 8.0 Hz, H-6) and 6.63 (d, J = 8.0 Hz, H-5), suggesting the presence of a 1,2,3,4-tetrasubstituted benzene ring. A spin system containing an oxygenated methine at δ_H 4.63 (H-3) and three aliphatic methylenes at δ_H 2.94 and 2.83 (H_ab-4), 1.96 (H₂-11) and 2.42 (H₂-12) was identified by their COSY correlations. In addition, three hydroxy groups at δ_H 12.21 (s, OH-13), 10.82 (s, OH-8) and 9.33 (s, OH-7) were observed. The HMBC correlations from H-5 to C-7 (δ_C 144.4) and C-9 (δ_C 108.4), from H-6 to C-8 (δ_C 149.9) and C-10 (δ_C 129.1), from OH-7 to C-6 (δ_C 121.6), C-7 and C-8, and from OH-8 to C-7, C-8 and C-9 established a benzene ring with two hydroxy groups at C-7 and C-8. The HMBC correlations from H-5 to C-4 (δ_C 31.2), from H_ab-4 to C-5 (δ_C 117.5), C-9 and C-10 as well as from H₂-11 and H₂-12 to C-13 (δ_C 173.8) indicated a 5-carboxypentyl chain to be attached at the C-10 position. Furthermore, the presence of an additional six-membered lactone ring fused with the benzene ring was confirmed by the HMBC correlations from H-5 and H-3 to C-1 (δ_C 169.6) combined with its molecular formula. The above findings concluded the structure of 15 as a new dihydroisocoumarin derivative as shown, for which the trivial name talumarin A is proposed.

Table 7.

¹H and ¹³C NMR Data for compounds 15 and 16.

No.	15 a		16 b
	δC, Type	δH (J in Hz)	δC, Type	δH (J in Hz)
1	169.6, C		169.5, C
3	79.3, CH	4.63, m	80.8, CH	4.63, m
4	31.2, CH2	2.94, dd (16.4, 3.6)	32.7, CH2	2.96, dd (16.1, 4.1)
		2.83, dd (16.4, 10.9)		2.87, dd (16.1, 10.4)
5	117.5, CH	6.63, d (8.0)	118.6, CH	6.65, d (8.0)
6	121.6, CH	7.01, d (8.0)	122.4, CH	7.01, d (8.0)
7	144.4, C		145.7, C
8	149.9, C		151.0, C
9	108.4, C		109.4, C
10	129.1, C		130.7, C
11	29.3, CH2	1.96, m	30.7, CH2	2.09, m
12	29.1, CH2	2.42, m	30.3, CH2	2.58, m
13	173.8, C		174.4, C
1′			65.3, CH2	4.11, t (6.6)
2′			31.6, CH2	1.63, m
3′			19.9, CH2	1.40, m
4′			13.7, CH3	0.95, t (7.4)
OH-7		9.33, s
OH-8		10.82, s
OH-13		12.21, s

^a Recorded at 300 MHz (¹H) and 75 MHz (¹³C) in DMSO-d₆. ^b Recorded at 300 MHz (¹H) and 75 MHz (¹³C) in CD₃OD.

Compound 16 shares almost identical UV absorptions with 15, suggesting the dihydroisocoumarin nature of the latter. The ESI-HRMS data established the molecular formula C₁₆H₂₀O₆ for 16. The NMR data of 16 were likewise similar to those of 15 (Table 7). However, signals of an additional n-butyl moiety were observed in the NMR spectra of 16, which was further confirmed by the COSY correlations between Me-4′ (δ_H 0.95)/H₂-3′ (δ_H 1.40), H₂-3′/H₂-2′ (δ_H 1.63), and H₂-2′/H₂-1′ (δ_H 4.11). In the HMBC spectrum, H₂-1′ showed correlation to C-12 (δ_C 174.4), indicating the attachment of the n-butyl moiety to C-12 through an ester bond. The remaining structure of 16 was found to be identical to that of 15 by detailed analysis of the 2D NMR data of 16.

To elucidate the absolute configuration of 15 and 16, TDDFT-ECD calculations were performed on a truncated model compound of 16 (R = Me). MMFF conformational search of the (R) enantiomer resulted in 42 conformers in a 21 kJ/mol energy window—DFT reoptimization of which yielded 8, 18 and 12 low-energy conformers at the B3LYP/6-31G(d) in vacuo, the B97D/TZVP PCM/MeCN and the CAM-B3LYP/TZVP PCM/MeCN levels. ECD calculations performed for each set of conformers gave moderate to good overall agreement with the experimental spectra of 15 and 16, allowing elucidation of the absolute configuration of 15 and 16 as (R) (Figure 4). The result of the ECD calculation corroborated with the previous ECD studies of the dihydroisocoumarin chromophore well [21,22,23], which located the n-π* ECD transition in the range of 250–270 nm and correlated the P/M helicity of the condensed heteroring with positive/negative n-π* Cotton effect, respectively. In the case of 15 and 16, the heteroring adopts M helicity with equatorial C-3 substituent, which gives rise to a negative n-π* Cotton effect at 263 and 262 nm, respectively.

Figure 4.

(a) structures and populations of the low-energy CAM-B3LYP/TZVP PCM/MeCN conformers (≥2%) of the truncated model compound of (R)-16; (b) experimental ECD spectra of 15 and 16 in MeCN compared with the Boltzmann-weighted PBE0/TZVP PCM/MeCN ECD spectrum of the truncated model compound of (R)-16 computed for the CAM-B3LYP/TZVP PCM/MeCN conformers. Bars represent the rotational strength values of the lowest-energy conformer.

The remaining known compounds were identified as lactone acid (4) [13], lactone diacid (5) [13], and aspergillumarin A (17) by comparison of their spectroscopic data with those in the literature [24,25].

2.2. Biological Activities

All isolated compounds (1–17) were evaluated for their cytotoxic activity against the L5178Y mouse lymphoma cell line. Only the two butenolide-resorcylide dimmers talarodilactones A and B (13 and 14) exhibited potent cytotoxicity with IC₅₀ (half maximal Inhibitory Concentration) values of 3.9 and 1.3 µM, respectively. Interestingly, the monomeric building blocks of 13 and 14, which were also isolated in this study, were inactive in comparison.

Moreover, all compounds were tested for their antimicrobial activities against Mycobacterium tuberculosis (H37Rv), Staphylococcus aureus (ATCC 25923) and Acinetobacter baumannii (ATCC BAA-1605) using a broth micro dilution assay. However, none of them was active at a dose of 25 µg/mL.

3. Discussion

In conclusion, analysis of the sponge-derived fungus T. rugulosus afforded seventeen lactone derivatives divided into four groups: five butenolides (1–5), seven resorcylide derivatives (6–12), two butenolide-resorcylide dimers (13 and 14) and three dihydroisocoumarins (15–17).Compounds 1–3, 11, 12 and 16 exhibited a butyl side chain in their structures. Many butylated natural products have been isolated from fungi [26,27,28] and plants [29,30]. In our study, all butylated derivatives were clearly detected in the original crude fungal extract by LC-MS. Moreover, the extraction and isolation processes did not involve n-BuOH. After incubation of the corresponding non-butylated compounds with n-BuOH for one week, no butylation of these compounds was detected by LC-MS. These results confirm that the butylated compounds isolated in the present study are natural compounds.

Numerous (3R)-resorcylide derivatives have been reported [17,18,19,20]. Some of them that were initially presumed to possess 3S configuration were later revised to have 3R configuration instead. However, all resorcylide derivatives (6–14) isolated in the present study were unambiguously assigned as (3S)-series by CD analysis. To the best of our knowledge, this is the first report of (3S)-resorcylide derivatives in nature. In addition, talarodilactones A and B (13 and 14) represent a new class of butenolide-resorcylide dimers that showed significant cytotoxicity against the L5178Y murine lymphoma cell line with IC₅₀ values of 3.9 and 1.3 µM, respectively.

4. Materials and Methods

4.1. General Experimental Procedures

Optical rotations were measured using a PerkinElmer-241 MC polarimeter (Waltham, MA, USA). Bruker AVANCE DMX 300 or 600 NMR spectrometers (Karlsruhe, Germany) were employed to record ¹H, ¹³C and 2D NMR spectra. A Thermo Finnigan LCQ Deca LC-MS system (San Jose, CA, USA) was used to analysis the crude extract. ESI-HRMS data were obtained by a FT-HRMS-Orbitrap (Thermo Finnigan, San Jose, CA, USA) mass spectrometer. A Dionex P580 system (Germering, Germany) coupled to a photodiode array detector (UVD340S) was used to perform HPLC analysis. A Europhere 10 C₁₈ (125 × 4 mm, L × ID, Knauer, Germany) was used for analytical separation. HPLC separation was carried out with a Lachrom-Merck Hitachi semi-preparative HPLC system (Darmstadt, Germany) (Pump L7100; UV detector L7400; column: Europhere 100 C₁₈, 300 × 8 mm, Knauer, Germany). Merck MN silica gel 60 M (0.04–0.063 mm, Dueren, Germany) or Sephadex LH-20 (Darmstadt, Germany) were applied for column chromatography as stationary phases. Pre-coated silica gel 60 F₂₅₄ plates (Merck) were used for TLC (Thin layer chromatography) analysis under detection at 254 and 366 nm and/or using anisaldehyde as spray-reagent. Spectral grade solvents were used for spectroscopic measurements while distilled solvents were used for column chromatographic separations. ECD spectra were recorded on a J-810 spectropolarimeter.

4.2. Fungal Material

The fungal strain was isolated from the healthy inner tissues of the sponge A. cannabina collected and identified by Semih Engin at Sığaçık-İzmir, Turkey. It was identified as Talaromyces rugulosus (GenBank accession No. KT071708) according to DNA amplification and sequencing of the fungal ITS region as described before [31].

4.3. Fermentation, Extraction and Isolation

The fungal strain was cultivated on solid rice medium in twelve 1L Erlenmeyer flasks (autoclaving 100 g of rice and 110 mL of 3.5% sea salt solution in each Erlenmeyer flask) at room temperature under static conditions. After 30 d, each flask was extracted overnight with ethyl acetate (3 × 400 mL), followed by filtration and evaporation. The obtained crude extract (5 g) was then partitioned between n-hexane and 90% aqueous MeOH. The MeOH extract was then subjected to vacuum liquid chromatography (VLC) on silica gel 60 using a gradient solvent elution system of n-hexane/EtOAc and CH₂Cl₂/MeOH to obtain 16 fractions (Fr.1 to Fr.16).

Fr.4 (509 mg) was fractionated on a Sephadex LH-20 column using MeOH as eluent to give ten subfractions (Fr.4-1 to Fr.4-10), among which subfraction Fr.4-6 was found to be the pure compound 6 (14.0 mg). Subfraction Fr.4-5 (119 mg) was further purified by semi-preparative HPLC with 60% MeOH/H₂O as eluting system to afford 1 (9.9 mg), 11 (2.1mg), 12 (2.5 mg) and 16 (2.0 mg).

Fr.5 (2.4 g) was subjected to a Sephadex LH-20 column with MeOH as mobile phase to yield nine subfractions (Fr.5-1 to Fr.5-9). Compounds 14 (3.9 mg) and 15 (3.1 mg) were obtained from subfraction Fr.5-6 (50.5 mg) by semi-preparative HPLC with a gradient of MeOH/H₂O as eluent. Subfraction Fr.5-4(570 mg) was chromatographed over a Sephadex LH-20 column using acetone to afford eight subfractions (Fr.5-4-1 to Fr.5-4-8). After purification by semi-preparative RP (Reversed Phase)-HPLC using a gradient elution of MeOH/H₂O, subfractions Fr.5-4-5 (18.9 mg) and Fr.5-4-6 (13.1 mg) yielded compounds 8 (1.2 mg) and 4 (6.2 mg) ,respectively. Subfraction Fr.5-3 (788 mg) was fractionated over a Sephadex LH-20column using MeOH as eluent to give five subfractions (Fr.5-3-1 to Fr.5-3-5). SubfractionFr.5-3-2 (47.2 mg) was purified by semi-preparative RP-HPLC using a gradient elution of MeOH/H₂O to yield 17 (9.3 mg). Following the same procedure as described for Fr.5-3-2, compounds 2 (2.2 mg) 3 (16.7 mg) and 5 (2.4 mg) were obtained from Fr.5-3-3 (71.5 mg) while compounds 7 (1.8 mg), 9 (1.5 mg), 10 (1.4 mg) and 13 (1.6 mg) were obtained from Fr.5-3-4 (53.7 mg).

Lactone acid n-butyl ester (1): brown amorphous solid; ${\left[\mathsf{\alpha }\right]}_{\mathrm{D}}^{23}$ +2 (c 0.54, EtOH); UV λ_max 207, 217 and 288 nm; ECD {MeCN, λ [nm] (Δε), c = 3.29 × 10⁻⁴ M} 281 (+0.30), 231sh (−0.07); ¹H and ¹³C NMR, see Table 1; ESI-HRMS m/z 327.1206 [M + Na]+ (calcd. for C₁₇H₂₀O₅Na, 327.1203).

4-Methoxylactone acid n-butyl ester (2): brown amorphous solid; ${\left[\mathsf{\alpha }\right]}_{\mathrm{D}}^{23}$ −4 (c 0.33, EtOH); UV λ_max 203, 220 and 292 nm; ECD {MeCN, λ [nm] (Δε), c = 2.47 × 10⁻⁴ M} 263 (−0.32), 222 (−0.76), 210 (+0.16); ¹H and ¹³C NMR, see Table 1; ESI-HRMS m/z 357.1308 [M + Na]+ (calcd. for C₁₈H₂₂O₆Na, 357.1309).

Lactone diacid 7-O-n-butyl ester (3): brown amorphous solid; ${\left[\mathsf{\alpha }\right]}_{\mathrm{D}}^{23}$ −4 (c 0.53, EtOH); UV λ_max 204, 220 and 292 nm; ¹H and ¹³C NMR, see Table 1; ESI-HRMS m/z 371.1101 [M + Na]+ (calcd. for C₁₈H₂₀O₇Na, 371.1101).

Lactone diacid (5): brown amorphous solid; ${\left[\mathsf{\alpha }\right]}_{\mathrm{D}}^{23}$ −3 (c 0.53, EtOH); UV λ_max 219 and 292 nm; ECD {MeCN, λ [nm] (Δε), c = 3.51 × 10⁻⁴ M} 307 (+0.03), 262 (−0.17), 238 (+0.02), 226 (−0.05), 209 (+0.16), 198 (−0.05); LC-MS m/z 315.0 [M + Na]⁺, 291.3 [M − H]⁻.

(3S)-cis-Resorcylide (6): brown amorphous solid; ${\left[\mathsf{\alpha }\right]}_{\mathrm{D}}^{23}$ +2 (c 0.10, MeOH); UV λ_max 216, 266 and 303 nm; ECD {MeCN, λ [nm] (Δε), c = 3.88 × 10⁻⁴ M} 316 (+2.41), 288sh (−1.37), 262 (−9.35), 230sh (+0.42), 211sh (+5.08), 198 (+6.90); ¹H and ¹³C NMR, see Table 2; ESI-HRMS m/z 291.1233 [M + H]⁺ (calcd. for C₁₆H₁₉O₅, 291.1227).

(3S,7S)-7-Hydroxyresorcylide (7): brown amorphous solid; ${\left[\mathsf{\alpha }\right]}_{\mathrm{D}}^{23}$ +18 (c 0.11 MeOH); UV λ_max 212, 264 and 302 nm; ECD {MeCN, λ [nm] (Δε), c = 2.27 × 10⁻⁴ M} 306 (+1.11), 286sh (+0.15), 261 (−5.71), 228sh (−1.99), 212 (+5.82); ¹H and ¹³C NMR, see Table 2; ESI-HRMS m/z 309.1333 [M + H]⁺ (calcd. for C₁₆H₂₁O₆, 309.1333).

(3S,7R)-7-Hydroxyresorcylide (8): brown amorphous solid; ${\left[\mathsf{\alpha }\right]}_{\mathrm{D}}^{23}$ −8 (c 0.13 MeOH); UV λ_max 213, 263 and 302 nm; ECD {MeCN, λ [nm] (Δε), c = 3.24 × 10⁻⁴ M} 308 (+0.28), 285sh (−0.54), 260 (−3.33), 228sh (−1.81), 211 (+5.46); ¹H and ¹³C NMR, see Table 2; ESI-HRMS m/z 309.1327 [M + H]⁺ (calcd. for C₁₆H₂₁O₆, 309.1333).

(3S,7S)-7-Methoxyresorcylide (9): brown amorphous solid; ${\left[\mathsf{\alpha }\right]}_{\mathrm{D}}^{23}$ +4 (c 0.13, MeOH); UV λ_max 213, 263 and 304 nm; ECD {MeCN, λ [nm] (Δε), c = 3.72 × 10⁻⁴ M} 302 (+1.62), 284sh (+0.78), 260 (−6.22), 228sh (−1.53), 212 (+3.34), 191 (−5.60); ¹H and ¹³C NMR, see Table 3; ESI-HRMS m/z 323.1495 [M + H]⁺ (calcd. for C₁₇H₂₃O₆, 323.1489.

(3S,7R)-7-Methoxyresorcylide (10): brown amorphous solid; ${\left[\mathsf{\alpha }\right]}_{\mathrm{D}}^{23}$ −8 (c 0.12, MeOH); UV λ_max 213, 265 and 298 nm; ECD {MeCN, λ [nm] (Δε), c = 1.86 × 10⁻⁴ M} 308 (+0.50), 287sh (−0.79), 260 (−5.47), 228sh (−2.77), 211 (+9.62); ¹H and ¹³C NMR, see Table 3; ESI-HRMS m/z 323.1493 [M + H]⁺ (calcd. for C₁₇H₂₃O₆, 323.1489).

(3S,7S)-7-O-n-Butylresorcylide (11): brown amorphous solid; ${\left[\mathsf{\alpha }\right]}_{\mathrm{D}}^{23}$ +5 (c 0.20, MeOH); UV λ_max 213, 265 and 303 nm; ECD {MeCN, λ [nm] (Δε), c = 1.58 × 10⁻⁴ M} 302 (+6.88), 284sh (+3.87), 261 (−21.91), 230sh (−5.75), 212 (+16.12), 191 (−22.28); ¹H and ¹³C NMR, see Table 4; ESI-HRMS m/z 365.1964 [M + H]⁺ (calcd. for C₂₀H₂₉O₆, 365.1959).

(3S,7R)-7-O-n-Butylresorcylide (12): brown amorphous solid; ${\left[\mathsf{\alpha }\right]}_{\mathrm{D}}^{23}$ −17 (c 0.20, MeOH); UV λ_max 213, 264 and 303 nm; ECD {MeCN, λ [nm] (Δε), c = 2.88 × 10⁻⁴ M} 309 (+0.23), 287sh (−0.74), 260 (−4.38), 227sh (−2.93), 210 (+8.23); ¹H and ¹³C NMR, see Table 4; ESI-HRMS m/z 365.1961 [M + H]⁺ (calcd. for C₂₀H₂₉O₆, 365.1959).

Talarodilactone A (13): brown amorphous solid; ${\left[\mathsf{\alpha }\right]}_{\mathrm{D}}^{23}$ +9 (c 0.21, MeOH); UV λ_max 212, 269 and 291 nm; ECD {MeCN, λ [nm] (Δε), c = 1.68 × 10⁻⁴ M} 296sh (+1.05), 284 (+1.11), 260 (−4.22), 226sh (−1.51), 209 (+3.86); ¹H and ¹³C NMR, see Table 6; ESI-HRMS m/z 619.1794 [M + Na]⁺ (calcd. for C₃₁H₃₂O₁₂Na, 619.1786).

Talarodilactone B (14): brown amorphous solid; ${\left[\mathsf{\alpha }\right]}_{\mathrm{D}}^{23}$ −22 (c 0.21, MeOH); UV λ_max 212, 268 and 291 nm; ECD {MeCN, λ [nm] (Δε), c = 2.49 × 10⁻⁴ M} 298 (+0.79), 285sh (+0.31), 259 (−5.69), 235 (+0.25), 225 (−4.24), 208 (+11.95); ¹H and ¹³C NMR, see Table 6; ESI-HRMS m/z 605.1625 [M + Na]⁺ (calcd. for C₃₀H₃₀O₁₂Na, 605.1629).

Talumarin A (15): brown amorphous solid; ${\left[\mathsf{\alpha }\right]}_{\mathrm{D}}^{23}$ −18 (c 0.27, CHCl₃); UV λ_max 220, 258 and 334 nm; ECD {MeCN, λ [nm] (Δε), c = 9.91 × 10⁻⁵ M} 324 (−0.35), 263 (−1.36), 242 (+1.20), 226sh (+0.75), 207 (−5.24); ¹H and ¹³C NMR, see Table 7; ESI-HRMS m/z 275.0528 [M + Na]⁺ (calcd. for C₁₂H₁₂O₆Na, 275.0526).

Talumarin B (16): brown amorphous solid; ${\left[\mathsf{\alpha }\right]}_{\mathrm{D}}^{23}$ −32 (c 0.27, CHCl₃); UV λ_max 221, 258 and 330 nm; ECD {MeCN, λ [nm] (Δε), c = 7.09 × 10⁻⁵ M} 327 (−0.26), 262 (−1.22), 243 (+0.75), 226sh (+0.17), 206 (−3.57); ¹H and ¹³C NMR, see Table 7; ESI-HRMS m/z 331.1151 [M + Na]⁺ (calcd. for C₁₆H₂₀O₆Na, 331.1151).

4.4. Computational Section

Geometry optimizations (B3LYP/6-31G(d) in vacuo, B97D/TZVP [32,33] and CAM-B3LYP/TZVP [34] with PCM solvent model for MeCN), and TDDFT calculations were performed with Gaussian 09 using various functionals (B3LYP, BH&HLYP, CAM-B3LYP and PBE0) and the TZVP basis set [35]. ECD spectra were generated as the sum of Gaussians with 2400 and 3000 cm⁻¹ half-height width (corresponding to c.a. 14 and 17 nm at 240 nm), using dipole-velocity computed rotational strengths [36]. Mixed torsional/low mode conformational searches were carried out by means of the Macromodel 10.8.011 software (New York, NY, USA) using Merck Molecular Force Field (MMFF) with implicit solvent model for CHCl₃ applying a 21 kJ/mol energy window [37]. Boltzmann distributions were estimated from the B3LYP, B97D and CAM-B3LYP energies. In the case of the B3LYP/6-31G(d) in vacuo level, ZPVE corrections were applied. The MOLEKEL software package (New York, NY, USA) was used for visualization of the results [38].

4.5. Cytotoxicity Assay

Cytotoxicity against the L5178Y murine lymphoma cell line was tested using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. The depsipeptide kahalalide F was used as positive control [39].

4.6. Antimicrobial Assay

The inoculum was obtained by direct colony suspension preparation. Antibacterial activities against Mycobacterium tuberculosis (H37Rv), Staphylococcus aureus (ATCC 25923) and Acinetobacter baumannii (ATCC BAA-1605) were evaluated by broth micro dilution methodology as stated by the recommendations of the Clinical and Laboratory Standards Institute (CLSI) [40].

Supplementary Materials

The following are available online at www.mdpi.com/1660-3397/15/11/359/s1, ESI-HRMS, 1D and 2D NMR spectra of all the new compounds as well as ECD spectra of compounds 1, 2, 5–16 and ECD calculations for compound 9.

Author Contributions

Lisa Küppers and Weaam Ebrahim performed the experiments of extraction and isolation; Mona El-Neketi prepared the manuscriptand contributed to part of the data analysis; Ferhat C. Özkaya and Weaam Ebrahim isolated, purified and identified the fungal strain; Attila Mándi and Tibor Kurtán preformed the ECD calculations; Werner E. G. Müller carried out the cytotoxicity assay; Raha S. Orfali contributed to part of the data analysis; Rudolf Hartmann measured the 1D and 2D NMR spectra; Wenhan Lin and Weiguo Song contributed to part of the structure elucidation; Zhen Liu and Peter Proksch supervised the research work and revised the manuscript.

Conflicts of Interest

The authors declare no conflict of interest.